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Recombinant Rabbit Monoclonal
Anti-AFP Rabbit mAb
Catalog Number: PTM-6322
$ 300

Clone Number: JRMR-10774-25

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF FC

Reactivity: Human, Mouse

Synonyms: Alpha-1-fetoprotein, HPAFP, alpha-fetoprotein

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Alpha-1-fetoprotein, HPAFP, alpha-fetoprotein
UniProt ID

P02771

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse
IHC-P 1:100 - 1:1000 Human
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

AFP is a major plasma protein expressed at high concentrations during fetal development; however, concentrations decrease drastically after birth. AFP is reactivated by approximately 80% of HCC. Like PSA, levels of serum AFP are an important diagnostic tool for detection of HCC. When hepatocytes regenerate or proliferate, AFP concentrations may increase. Elevated AFP concentrations may be observed in cirrhotic patients, during a flare-up of chronic hepatitis, or during the recovery phase postacute hepatitis. Additionally, AFP is commonly used as a tumor marker for hepatocellular carcinoma (HCC) or germ cell (nonseminoma) carcinoma.

Cellular location

Secreted

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: Mouse placenta
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 69 kDa
Observed band size: 69 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HepG2
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 69 kDa
Observed band size: 69 kDa
IHC-P

Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6322

Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6322
ICC/IF

Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6322
FC

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6322

Research Use

For research use only, not for use in diagnostic procedures.

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