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Custom Antibody Development

PTMab® provides an all-in-one antibody generation package, including immunogen design & synthesis, immunization, antibody purification, and validation, to help achieve your research goals. Besides the standard unmodified antibodies, we offer a proprietary custom antibody generation service against all the post-translational modifications (PTMs). As the pioneer in PTM reagents, we have in-house developed 400+ PTM antibodies covering over 30 PTM types, including novel acylations such as lysine lactylation, crotonylation, and succinylation. We have also developed over 1000 recombinant rabbit monoclonal antibodies based on our robust high throughput antibody generation platform. We leverage our extensive experience to provide you custom antibody solutions with exquisite specificity and exceptional sensitivity.

Custom Antibody Solutions
  • Custom Rabbit Polyclonal Antibody Generation (Unmodified & PTM-Specific)
    Standard Development Process
    Phases Package A
    Unmodified Antibody    		 Package B
    PTM-Specific Antibody    		 Deliverables

    Antigen Preparation

    (2~3 weeks)

    2 Antigenic polypeptides

    QC by in-house mass spectrometry facility

    2 Antigenic polypeptides

    1 Unmodified control polypeptide

    QC by in-house mass spectrometry facility

    1 mg each peptide

    Immunization

    (8~11 weeks)

    4 Rabbits

    1 primary injection + 5 booster injections

    6 Rabbits

    1 primary injection + 5 booster injections

    /

    Serum Screening &

    Purification

    (1~2 weeks)

    Serum primary screening by ELISA and WB.Affinity purification against Protein A and antigen.

    Serum primary screening by ELISA and WB.Affinity purification against Protein A and antigen, followed by depletion in an unmodified polypeptide affinity column

    /

    Validation

    (1~2 weeks)

    QC Standards:

    1. Minimum antibody titer of 1:50,000 as determined by ELISA;

    2. Test one of the following

    applications: WB, ICC, or IHC.

    QC Standards:

    1. Minimum antibody titer of 1:50,000 as determined by ELISA;

    2. Dot Blot: Recognition of the modified antigen polypeptide at a minimum of 20 ng. The antibody should demonstrate at least 10-fold higher selectivity for the modified peptide over the unmodified peptide.

    3. Endogenous target protein detected by WB.

    2 mg antibody and development report.
    Featured Case Studies
    • Molecular Plant
      Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat. 2019.
      PMID
      31606466
      DOI
      10.1016/j.molp.2019.09.010
      Custom Antibody Product
      Anti-PstGSRE1 Rabbit pAb, Anti-TaLOL2 Rabbit pAb
      Reactivity
      Wheat
      Application
      WB
    • Plant Biotechnology Journal
      Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum. 2021
      PMID
      33368971
      DOI
      10.1111/pbi.13533.
      Custom Antibody Product
      Anti-DgTIL1 Rabbit pAb, Anti-Crotonyl-DgTIL1 (Lys72) Rabbit pAb
      Reactivity
      Chrysanthemum, Tobacco
      Application
      WB
    • Cancer Cell
      Direct Phosphorylation and Stabilization of MYC by Auror B Kinase Promote T-cell Leukemogenesis. 2020.
      PMID
      32049046
      DOI
      10.1016/j.ccell.2020.01.001
      Custom Antibody Product
      Anti-Phospho-MYC (Ser67) Rabbit pAb
      Reactivity
      Human, Mouse
      Application
      WB
    • Nature Communications
      Isonicotinylation is a histone mark induced by the anti-tuberculosis first-line drug isoniazid. 2021
      PMID
      34545082
      DOI
      10.1038/s41467-021-25867-y
      Custom Antibody Product
      Anti-Kinic Rabbit pAb
      Reactivity
      Human, Mouse
      Application
      WB, IHC-P, IF
    • Nature Communications
      Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia. 2019
      PMID
      31699991
      DOI
      10.1038/s41467-019-12960-6
      Custom Antibody Product
      Anti-Acetyl-AML1-ETO (Lys43) Rabbit pAb, Anti-Mono-Methyl-AML1-ETO (Lys43) Rabbit pAb
      Reactivity
      Human
      Application
      WB，ChIP-seq
  • Custom Recombinant Rabbit Monoclonal Antibody Generation (Unmodified & PTM-Specific)
    Standard Development Process
    Phases Package A

    Unmodified Antibody

    		Package B

    PTM-Specific Antibody

    		 Deliverables

    Antigen Preparation

    (2~3 weeks)

    2 Antigenic polypeptides

    QC by in-house mass spectrometry facility

    2 Antigenic polypeptides

    1 Unmodified control polypeptide

    QC by in-house mass spectrometry facility

    1 mg each peptide

    Immunization

    (8~11 weeks)

    4 Rabbits

    1 primary injection + 5 booster injections

    6 Rabbits

    1 primary injection + 5 booster injections

    		 /

    Serum Screening & Purification

    (1~2 weeks)

    Serum primary screening by ELISA and WB.

    Affinity purification against Protein A and antigen

    Serum primary screening by ELISA and WB.

    Affinity purification against Protein A and antigen, followed by depletion in an unmodified polypeptide affinity column

    		 /

    B Cell Panning & Screening

    (2 weeks)

    B cell panning and ELISA screening for positive clones

    		B cell panning and ELISA screening for positive clones /

    Antibody Cloning & Recombinant Antibody Production

    (7-8 weeks)

    		Select the best positive clone for sequencing, IgG cloning and recombinant antibody production Select the best positive clone for sequencing, IgG cloning and recombinant antibody production /

    Validation

    (1~2 weeks）

    QC Standards:

    1. Minimum antibody titer of 1:50,000 as determined by ELISA;

    2. Endogenous target protein detected by WB.

    QC Standards:

    1. Minimum antibody titer of 1:50,000 as determined by ELISA;

    2. Dot Blot: Recognition of the modified antigen polypeptide at a minimum of 20 ng. The antibody should demonstrate at least 10-fold higher selectivity for the modified peptide over the unmodified peptide.

    3. Endogenous target protein detected by WB.

    		2 mg antibody and development report

The ability to design and produce appropriate antigen is central to PTMab®’s production of high quality antibodies. Our bioinformatics analysis program will search and assess your sequence of interest to identify the best candidate peptides that optimize the hydrophobicity, specificity, and antigenicity based on epitope mapping, cross-reactivity with other structurally similar peptides, and 3D confirmation analysis.

Contact our scientist today to discuss your antibody needs here!

Custom Antibody Quote Request
Please complete this form with detailed information for us to evaluate the project and provide you the best custom antibody solution.
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Custom Antibody of Interest
Target Information：
UniProt ID
Please provide the amino acid sequence here if UniProt ID is not available:
PTM Type
Please provide the chemical structure here for novel PTM types
Please provide the chemical structure here for novel PTM types
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