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Recombinant Rabbit Monoclonal
Anti-AMPK alpha 1 + AMPK alpha 2 Rabbit mAb
Catalog Number: PTM-6739
$ 280

Clone Number: JMMR-3405

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: PRKAA1, AMPK1, Acetyl-CoA carboxylase kinase, ACACA kinase, HMGCR kinase, ACACA kinase, HMGCR kinase, AMPK subunit alpha-2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms PRKAA1, AMPK1, Acetyl-CoA carboxylase kinase, ACACA kinase, HMGCR kinase, ACACA kinase, HMGCR kinase, AMPK subunit alpha-2
UniProt ID

Q13131, P54646

Immunogen
MW (kDa) 62
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

AMPK alpha is the catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.

Cellular location

Nucleus, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate: 1: HeLa, 2: HepG2
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 62 kDa
Observed band size: 62 kDa

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy
    Year 2024
    Journal Cell Reports
    Authors Shuang Li, et al.
    Applications Unspecified
    Reactivity