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Recombinant
Anti-BMI1 Mouse mAb
Catalog Number: PTM-5709
$ 285

Clone Number: 12233

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse

Synonyms: Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, PCGF4, RNF51

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2a
Conjugate Unconjugated
Synonyms Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, PCGF4, RNF51
UniProt ID

P35226

Immunogen Synthetic peptide derived from human polycomb complex protein BMI-1
MW (kDa) 37
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

BMI1 is a protein that is encoded by the BMI1 gene (B cell-specific Moloney murine leukemia virus integration site 1) in human. BMI1 has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 gene knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Recently it has been reported BMI1 is rapidly recruited to sites of DNA damage and it sustains for over 8h. BMI1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: MCF7, 2: K-562, 3: A20, 4: NIH/3T3
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 37 kDa
Observed band size: 40 kDa

Research Use

For research use only, not for use in diagnostic procedures.