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Recombinant
Anti-CREB Mouse mAb
Catalog Number: PTM-5595
$ 290

Clone Number: 10722

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: CREB1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms CREB1
UniProt ID

P16220

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse, Rat
IHC-P 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: PC-12, 3: C2C12, 4: HEK_x0002_293
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 43 kDa
Observed band size: 43 kDa

IHC-P

Tissue: Human lung squamous cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5595

Research Use

For research use only, not for use in diagnostic procedures.

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