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Recombinant Rabbit Monoclonal
Anti-Ctip2/BCL11B Rabbit mAb
Catalog Number: PTM-6156
$ 285

Clone Number: JRMR-10395-3

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P FC

Reactivity: Human, Mouse, Rat

Synonyms: BCL11B, RIT1, Ctip2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms BCL11B, RIT1, Ctip2
UniProt ID

Q9C0K0

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Mouse, Rat
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Ctip2 is the Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours atroom temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate: 1: Jurkat, 2: MOLT-4, 3: Mouse
hippocampus, 4: Rat hippocampus
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 96 kDa
Observed band size: 120 - 130 kDa

IHC-P

Tissue: Mouse cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour atroom temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: Color brown is the positive signalfor PTM-6156

Tissue: Rat spleen
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour atroom temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: Color brown is the positive signalfor PTM-6156

FC

Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6156

Research Use

For research use only, not for use in diagnostic procedures.