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Recombinant Rabbit Monoclonal
Anti-Keratin 16 Rabbit mAb
Catalog Number: PTM-5340
$ 290

Clone Number: JRMR-10024-29H2L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF

Reactivity: Human

Synonyms: CK-16, Cytokeratin-16, KRT16

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms CK-16, Cytokeratin-16, KRT16
UniProt ID

P08779

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human
IHC-P 1:200 - 1:1000 Human
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Cellular location

Membrane, cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 51 kDa
Observed band size: 51 kDa

IHC-P

Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5340

Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5340

ICC/IF

Cell line: A431
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5340

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 290
    Recombinant Rabbit Monoclonal
    Anti-Keratin 16 Rabbit mAb
    Catalog #
    PTM-5341
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P