中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-ELP4 Rabbit mAb
Catalog Number: PTM-6385
$ 280

Clone Number: JRMR-10680-46

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: C11orf19, PAXNEB

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms C11orf19, PAXNEB
UniProt ID

Q96EB1

Immunogen Recombinant human ELP4 fragment
MW (kDa) 53, 59
Specificity Anti-ELP4 Rabbit mAb recognizes endogeneous level of ELP4 protein.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human
IP 1:25 - 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Elongator Acetyltransferase Complex Subunit 4 (ELP4) is a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. The elongator complex catalyzes formation of carboxy methyluridine in the wobble base at position 34 in tRNAs. Diseases associated with ELP4 include Aniridia 2 and Coloboma of Optic Nerve. Among its related pathways are Mesodermal Commitment Pathway and Chromatin organization.

Cellular location

Cell nucleus and cytosol

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 47 kDa
Observed band size: 53, 59 kDa (two isoforms differ from the canonical sequence)
ICC/IF

Cell line: HepG2
Fixation: 100% methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for PTM-6385
IP

IP of HeLa cells extracts
IP Ab incubation: PTM-6385, 4°C overnight, 1:50 dilution
WB Primary Ab incubation: PTM_x0002_6385, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5% Input
2: IP with PTM-6385
3: IP with Rabbit monoclonal IgG Isotype
Observed band size: 53 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.