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Recombinant
Anti-FAK Mouse mAb
Catalog Number: PTM-5717
$ 280

Clone Number: 12303

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse

Synonyms: PTK2, FAK1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2c
Conjugate Unconjugated
Synonyms PTK2, FAK1
UniProt ID

Q05397

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates.

Cellular location

Cell junction, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: SKOV-3, 3: 4T1
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 110 kDa
Observed band size: 110 kDa

Research Use

For research use only, not for use in diagnostic procedures.