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Recombinant Rabbit Monoclonal
Anti-GNB2 Rabbit mAb
Catalog Number: PTM-5959
$ 290

Clone Number: 13225

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2, Transducin beta chain 2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2, Transducin beta chain 2
UniProt ID

P62879

Immunogen Synthetic peptide derived from human GNB2
MW (kDa) 32
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, SH-SY5Y, Raw264.7, Rat spleen
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 32 kDa
Observed band size: 32 kDa

ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5959

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 290
    Recombinant Rabbit Monoclonal
    Anti-GNB2 Rabbit mAb
    Catalog #
    PTM-5649
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag