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Recombinant Rabbit Monoclonal
Anti-HNF-1B Rabbit mAb
Catalog Number: PTM-6320
$ 290

Clone Number: JRMR-10794-28

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: TCF-2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms TCF-2
UniProt ID

P35680

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:10000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Hepatocyte nuclear factor 1-beta (HNF-1B) is a transcription factor which binds to specific regions of nucleic acid. HNF-1B, which plays critical roles in the regulation of glucose transport and metabolism, glucose-stimulated insulin secretion, and also in the embryonic development and later function of the pancreas, kidneys, genitourinary tract, liver, and the biliary tree.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: Jurkat
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 61 kDa
Observed band size: 61 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Mouse liver, 2: Rat liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 61 kDa
Observed band size: 61 kDa

Research Use

For research use only, not for use in diagnostic procedures.