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Anti-IgM Rabbit pAb
Catalog Number: PTM-6648
$ 290

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB ICC/IF FC

Reactivity: Human

Synonyms: IGHM

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms IGHM
UniProt ID

P01871

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In the case of humans and other mammals that have been studied, the spleen, where plasmablasts that are responsible for antibody production reside, is the major site of specific IgM production.

Cellular location

Membrane, Secreted

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate: 1: MOLT-4 (Negative control), 2:Daudi, 3: Raji, 4: Ramos
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 49 kDa
Observed band size: 75 kDa

ICC/IF

Cell line: Raji
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6648

FC

Cell line: Raji
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation
with primary and secondary antibodies(Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6648

Research Use

For research use only, not for use in diagnostic procedures.

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    Catalog #
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    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications:
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