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Recombinant
Anti-Keratin 14 Mouse mAb
Catalog Number: PTM-5943
$ 280

Clone Number: 12691

Host: Mouse Clonality: Recombinant Monoclonal

Applications: IHC-P ICC/IF

Reactivity: Human

Synonyms: CK-14, KRT14, K14, Cytokeratin-14

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG3
Conjugate Unconjugated
Synonyms CK-14, KRT14, K14, Cytokeratin-14
UniProt ID

P02533

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
IHC-P 1:50 - 1:200 Human
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Cellular location

Cytoplasm, Intermediate filament, Keratin, Nucleus

Images
IHC-P

Tissue: Human esophagus
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5943

Tissue: Human prostatic hyperplasia
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5943

ICC/IF

Cell line: A431
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5943

Research Use

For research use only, not for use in diagnostic procedures.

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