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Recombinant
Anti-LDHB Mouse mAb
Catalog Number: PTM-5869
$ 290

Clone Number: 11965

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human, Rat

Synonyms: LDH-B, L-lactate dehydrogenase B chain

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms LDH-B, L-lactate dehydrogenase B chain
UniProt ID

P07195

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:5000 - 1:20000 Human, Rat
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.

Cellular location

Cytoplasm, Membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:20000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, SH-SY5Y
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: Jurkat, Rat brain
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 35 kDa
Observed band size: 35 kDa

ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5869

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • Epigenetic silencing of LDHB promotes hepatocellular carcinoma by remodeling the tumor microenvironment
    Year 2024
    Journal CANCER IMMUNOLOGY IMMUNOTHERAPY
    Authors Zhang Peng, et al.
    Applications Unspecified
    Reactivity