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Recombinant Rabbit Monoclonal
Anti-MAP1LC3A Rabbit mAb
Catalog Number: PTM-5896
$ 290

Clone Number: 11868

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: MAP1A/MAP1B LC3 A

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms MAP1A/MAP1B LC3 A
UniProt ID

Q9H492

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:10000 - 1:50000 Mouse, Rat
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. Two transcript variants encoding different isoforms have been found for this gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. MAPLC3A is one of the mammalian homologues of yeast ATG8, an important marker and effector of autophagy.

Cellular location

Cytoplasm, Membrane, Microtubule

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:50000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Rat brain, 2: Mouse brain, 3: Mouse
cerebellum, 4: Rat cerebellum
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 14 kDa
Observed band size: 16 kDa

IHC-P

Tissue: Human brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5896

ICC/IF

Cell line: SH-SY5Y
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°Covernight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5896

Research Use

For research use only, not for use in diagnostic procedures.