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Recombinant
Anti-Mre11 Mouse mAb
Catalog Number: PTM-6851
$ 280

Clone Number: JMMR-3573

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Meiotic recombination 11 homolog 1, HNGS1, MRE11A

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms Meiotic recombination 11 homolog 1, HNGS1, MRE11A
UniProt ID

P49959

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Mre11, originally described in genetic screens from the yeast Saccharomyces cerevisiae in which mutants were defective in meiotic recombination, is a central part of a multisubunit nuclease composed of Mre11, Rad50 and Nbs1 (MRN). The MRN complex plays a critical role in sensing, processing and repairing DNA double strand breaks. Defects lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage. Hypomorphic mutations of Mre11 are found in ataxia-telangiectasia-like disease (ATLD), with phenotypes similar to mutations in ATM that cause ataxia-telangiectasia (A-T). Cellular consequences of ATLD include chromosomal instability and defects in the intra-S phase and G2/M checkpoints in response to DNA damage. The MRN complex may directly activate the ATM checkpoint kinase at DNA breaks.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HeLa; 2: K562; 3: 293T
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 81 kDa
Observed band size: 85 kDa

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • Metabolic regulation of homologous recombination repair by MRE11 lactylation
    Year 2023
    Journal CELL
    Authors Yuping Chen, et al.
    Applications Unspecified
    Reactivity