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Recombinant
Anti-NSE Mouse mAb
Catalog Number: PTM-6449
$ 280

Clone Number: JMMR-6263

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Enolase 2, Neural enolase, Neuron-specific enolase

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2a
Conjugate Unconjugated
Synonyms Enolase 2, Neural enolase, Neuron-specific enolase
UniProt ID

P09104

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Neuron specific enolase (NSE), also known as enolase 2 (ENO2) or gamma-enolase, is an enzyme that is encoded by the ENO2 gene in human. NSE is a phosphopyruvate hydratase. NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Cellular location

Cytoplasm, Membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: U-87 MG, 2: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 47 kDa
Observed band size: 47 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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