Clone Number: 12635
Host: Mouse Clonality: Recombinant Monoclonal
Applications： WB ICC/IF
Reactivity: Human, Rat
Synonyms: UCHL1, UCH-L1, PGP 9.5
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG2a |
| Conjugate | Unconjugated |
| Synonyms | UCHL1, UCH-L1, PGP 9.5 |
| UniProt ID | |
| Immunogen | |
| MW (kDa) | |
| Specificity |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:1000 | Human, Rat |
| ICC/IF | 1:50 | Human |
| Purity | Protein G purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Cellular location
Cytoplasm, Endoplasmic reticulum, Membrane
| WB | |
|---|---|
|
|
Blocking buffer: 5% NFDM/TBST |
| ICC/IF | |
|---|---|
|
|
Cell line: SH-SY5Y |
Research Use
For research use only, not for use in diagnostic procedures.
