/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-PKC delta Rabbit mAb
Catalog Number: PTM-6206
$ 285

Clone Number: 11553

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: nPKC-delta, SDK1, PKCD, PRKCD

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms nPKC-delta, SDK1, PKCD, PRKCD
UniProt ID

Q05655

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

PKC-delta(Protein kinase C delta type) is an enzyme that is encoded by the PRKCD gene in human. PKC is a family of serine- and threonine-specific protein kinases that can be activated by the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC-delta is one of the PKC family members. Studies both in human and mice demonstrate that PKC-delta is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PKC-delta is also regulated by phosphorylation on various serine/threonine and tyrosine residues including Y311.

Cellular location

Cytoplasm, Membrane, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: Rat brain
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 78 kDa
Observed band size: 80 kDa

ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6206

Research Use

For research use only, not for use in diagnostic procedures.