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Recombinant
Anti-PPP3CB Mouse mAb
Catalog Number: PTM-5173
$ 290

Clone Number: 13409

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform (CNA beta), CALNA2, CALNB, CNA2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform (CNA beta), CALNA2, CALNB, CNA2
UniProt ID

P16298

Immunogen Synthetic peptide derived from human PPP3CB
MW (kDa) 59
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Mouse, Rat
IHC-P 1:50 - 1:100 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PP2BB) is an enzyme that in humans is encoded by the PPP3CB gene. This subunit may have a role in the calmodulin activation of calcineurin. It is a calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca2+-mediated signals. It can dephosphorylates and activates transcription factor NFATC1, and dephosphorylates and inactivates transcription factor ELK1.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 4℃ overnight
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 59 kDa
Observed band size: 59 kDa
IHC-P

ssue: Human brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5173

Research Use

For research use only, not for use in diagnostic procedures.

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