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Recombinant Rabbit Monoclonal
Anti-Phospho-AKT1 (Ser473) Rabbit mAb
Catalog Number: PTM-6649
$ 300

Clone Number: JRMR-10760-38-H0L0

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: RAC-alpha serine/threonine-protein kinase

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms RAC-alpha serine/threonine-protein kinase
UniProt ID

P31749

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein).

Cellular location

Nucleus, Cell membrane, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate: (-): Jurkat, (+): Jurkat+ Calyculin A(100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate:(-): MCF-7, (+): MCF-7+ Calyculin A(100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate:(-): HeLa, (+): HeLa+ Calyculin A(100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate:(-): NIH/3T3, (+): NIH/3T3+ CalyculinA(100ng/ml, 60min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate:(-): C6, (+): C6+ Calyculin A (100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate:1: Jurkat+ Calyculin A (100nM, 30min) ,then the membrane treated with Lambda Protein Phosphatase (400 U/ml) at 30°C for 1hour; 2: Jurkat+ Calyculin A (100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 56 kDa
Observed band size: 56, 60 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 290
    Recombinant Rabbit Monoclonal
    Anti-AKT1 Rabbit mAb
    Catalog #
    PTM-6071
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • N1-methyladenosine RNA methylation patterns are associated with an increased risk to biochemical recurrence in prostate cancer and serve as a potential novel biomarker for patient stratification
    Year 2024
    Journal INTERNATIONAL IMMUNOPHARMACOLOGY
    Authors Yulin Deng, et al.
    Applications Unspecified
    Reactivity
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