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Recombinant Rabbit Monoclonal
Anti-Phospho-CREB (Ser133) Rabbit mAb
Catalog Number: PTM-5751
$ 300

Clone Number: JRMR-10392-60

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P FC

Reactivity: Human

Synonyms: CREB1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms CREB1
UniProt ID

P16220 - 1

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
IHC-P 1:50 - 1:100 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa + Calyculin A (100nM, 15min)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 37 kDa
Observed band size: 37 kDa

IHC-P

Tissue: Human lung adenocarcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5751

FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5751

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
  • Baicalein Ameliorates Insulin Resistance of HFD/STZ Mice through Activating PI3K/AKT Signal Pathway of Liver and Skeletal Muscle in a GLP-1R-Dependent Manner
    Year 2024
    Journal Antioxidants
    Authors Na Liu, et al.
    Applications Unspecified
    Reactivity