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Recombinant Rabbit Monoclonal
Anti-Pin1 Rabbit mAb
Catalog Number: PTM-6544
$ 280

Clone Number: JRMR-11025-13

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF FC

Reactivity: Human, Mouse, Rat

Synonyms: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1 (PPIase Pin1)

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1 (PPIase Pin1)
UniProt ID

Q9QUR7

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:10000 Human, Mouse, Rat
ICC/IF 1:50 Human, Mouse
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Pin1 is a member of the parvulin family of peptidyl-prolyl isomerases (PPIase). It has been implicated in the G2/M transition of the mammalian cell cycle. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules.

Cellular location

Nucleus, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Mouse skin (Low expression), 2: Mouse brain
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Mouse spleen, 2: NIH/3T3, 3: PC_x0002_12, 4: MCF-7, 5: HeLa
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 18 kDa
Observed band size: 18 kDa
ICC/IF

Cell line: Hela
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-6544

Cell line: NIH-3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-6544
FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6544

Research Use

For research use only, not for use in diagnostic procedures.

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