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Recombinant
Anti-RPS12 Mouse mAb
Catalog Number: PTM-5763
$ 290

Clone Number: 13070

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: Small ribosomal subunit protein eS12, 40S ribosomal protein S12

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms Small ribosomal subunit protein eS12, 40S ribosomal protein S12
UniProt ID

P25398

Immunogen Synthetic peptide derived from human small ribosomal subunit protein eS12
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:10000-:30000 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:30000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, HepG2, N2a, BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 15 kDa
Observed band size: 15 kDa

ICC/IF

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5763

Research Use

For research use only, not for use in diagnostic procedures.