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Recombinant
Anti-S100A10 Mouse mAb
Catalog Number: PTM-6634
$ 280

Clone Number: JMMR-1272D

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF

Reactivity: Human

Synonyms: Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms Protein S100-A10, Calpactin I light chain, S100 calcium-binding protein A10, p10 protein
UniProt ID

P60903

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

S100A10 (also known as p11 or calpactin 1 light chain) forms a constitutive heterotetramer with annexin-A2 (ANXA2) and may act as a bridge between the plasma membrane and actin cytoskeleton via interactions with the plasma membrane (via ANXA2) and various protein partners such as the SNARE complex or actin. S100A10 has been hypothesized to play a critical role in neuronal signaling due to its interaction and regulation of neurotransmitter receptors and neuron-specific ion channels such as 5-HT1B, TRPV5, ASIC1, TASK1 and NaV1.8 . More recently, S100A10 has also been shown to modulate macrophage activation and invasion via its ability to bind and transmit receptor-like signals in response to plasminogen.

Cellular location

Plasma Membrane, Nucleus, secreted

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)
Lysate: 1: HT-29, 2: A431
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa

IHC-P

Tissue: Human cervical carcinoma
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6634

Tissue: Human prostatic hyperplasia
Section type: Formalin-fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6634

ICC/IF

Cell line: HT-29
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6634

Research Use

For research use only, not for use in diagnostic procedures.