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Recombinant Rabbit Monoclonal
Anti-STAT6 Rabbit mAb
Catalog Number: PTM-6057
$ 280

Clone Number: JRMR-10005(2)-33

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB ICC/IF FC

Reactivity: Human

Synonyms: IL-4 Stat

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms IL-4 Stat
UniProt ID

P42226

Immunogen Recombinant human STAT6 fragment
MW (kDa) 110
Specificity Anti-STAT6 Rabbit mAb recognizes endogeneous level of STAT6 protein.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Raji, 2: Daudi
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 94 kDa
Observed band size: 110 kDa

ICC/IF

Cell line: Hela
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6057

Cell line: Raji
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6057

FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM_x0002_6057

Research Use

For research use only, not for use in diagnostic procedures.

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    Anti-STAT6 Rabbit pAb
    Catalog #
    PTM-6805
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P