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Recombinant Rabbit Monoclonal
Anti-alpha-Synuclein Rabbit mAb
Catalog Number: PTM-6345
$ 280

Clone Number: JRMR-10752-22

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF FC

Reactivity: Human, Mouse, Rat

Synonyms: SNCA, NACP, PARK1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms SNCA, NACP, PARK1
UniProt ID

P37840

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse, Rat
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Alpha-synuclein may serve to integrate presynaptic signaling and membrane trafficking. Defects in this protein have been implicated in the pathogenesis of Parkinson disease. Alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease.

Cellular location

Cytoplasm, Membrane, Nucleus, Cell junction, Synapse, Secreted

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 14 kDa
Observed band size: 18 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: U-87 MG
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 14 kDa
Observed band size: 18 kDa
ICC/IF

Cell line: U-87 MG
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-6345
FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6345

Cell line: U-87 MG
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-6345

Research Use

For research use only, not for use in diagnostic procedures.

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