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Recombinant Rabbit Monoclonal
Anti-mtTFA Rabbit mAb
Catalog Number: PTM-6993
$ 280

Clone Number: JMMR-3540

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human

Synonyms: MitochondrialTranscription factor A, Mitochondrial transcription factor 1 (MtTF1), Transcription factor 6 (TCF-6), Transcription factor 6-like 2, TFAM, TCF6, TCF6L2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms MitochondrialTranscription factor A, Mitochondrial transcription factor 1 (MtTF1), Transcription factor 6 (TCF-6), Transcription factor 6-like 2, TFAM, TCF6, TCF6L2
UniProt ID

Q00059

Immunogen Synthetic peptide derived from human mitochondrial transcription factor A
MW (kDa) 29
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human
IHC-P 1:50 - 1:200 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Mitochondrial Transcription Factor A (mtTFA) also named as TFAM or aka TCF6, is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. mtTFA is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B. Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. mtTFA plays an essential role in the maintenance of mtDNA and thus, ATP production. mtTFA binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA. mtTFA attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia.

Cellular location

Mitochondrion

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)
Lysate: 1: HeLa, 2: MCF-7, 3: T-47D, 4: 293T
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 29 kDa
Observed band size: 24 kDa

IHC-P

Tissue: Human kidney
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:200
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6993

Tissue: Human ovarian carcinoma
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:200
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6993

Research Use

For research use only, not for use in diagnostic procedures.