Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB
Reactivity: Human, Mouse, Rat
Synonyms: H2BK15bhb
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H2BK15bhb |
| UniProt ID | |
| Immunogen | β-Hydroxybutylated human histone H2B (Lys15) peptide |
| MW (kDa) | 14 |
| Specificity | Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit pAb detects histone H2B only when it is β-hydroxybutyrylated at Lys15. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.
Cellular location
Nucleus
| Dot Blot | |
|---|---|
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Peptide amount: 1 ng, 4 ng, 16 ng |
| WB | |
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Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) |
Research Use
For research use only, not for use in diagnostic procedures.
