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Anti-β-Hydroxybutyryl-Histone H3 (Lys4) Mouse mAb
Catalog Number: PTM-1296
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications: WB IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K4bhb

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K4bhb
UniProt ID

P68431

Immunogen β-Hydroxybutylated human histone H3 (Lys4) peptide
MW (kDa) 15
Specificity Anti-β-Hydroxybutyryl-Histone H3 (Lys4) Mouse mAb detects histone H3 only when it is β-hydroxybutyrylated at Lys4.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IP 1:25 - 1:100 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided in the table below.

WB

Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

IP

IP of HeLa + sodium 3-hydroxybutyrate (30 mM, 4 hours) cells extracts
Lane 1: 5% Input
Lane 2: IP with PTM-1296 (1:25)
Lane 3: IP with PTM-1296 (1:100)
IP Ab incubation condition: PTM-1296, 4°C overnight, 1:25, 1:100 dilutions
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB Primary Ab incubation condition: PTM-1258 Anti-H3K4bhb Rabbit pAb, 2 hours at room temperature, 1:1000 dilution
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 17 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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