Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB ICC/IF IHC-P
Reactivity: All
Synonyms: Kbhb
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | Kbhb |
| UniProt ID | |
| Immunogen | β-Hydroxybutylated lysine peptides |
| MW (kDa) | Multiple |
| Specificity | Anti-β-Hydroxybutyllysine Rabbit mAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:1000 | All |
| ICC/IF | 1:50 | Human |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.
Cellular location
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| Dot Blot | |
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Peptide/protein amount: 10 ng |
| WB | |
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Lysates: HeLa, C2C12, COS, (-) HeLa, (+) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) |
| ICC/IF | |
|---|---|
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Samples: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) |
| IHC-P | |
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Tissue: Human kidney |
| IHC-P | |
|---|---|
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Tissue: Human placenta |
Research Use
For research use only, not for use in diagnostic procedures.
