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Anti-2-Hydroxyisobutyryl-Histone H4 (Lys5) Mouse mAb
Catalog Number: PTM-854
$ 435

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H4K5hib

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K5hib
UniProt ID

P62805

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ChIP 4 μg/5x106 cells Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone 2-hydroxyisobutyrylation marks either active promoters or potential enhancers.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (Left) HeLa±Sodium butyrate (30mM, 4hr), (Right) HeLa±Sodium pyruvate (100mM, 72hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa
ChIP

Cell type: Hela + sodium butyrate (30 mM, 4 hr)
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 4 μg, 12 μg
Beads type and amount per IP: 50 μl of
Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 1 μg
of normal mouse IgG as a negative control. Real
time quantitative PCR was performed on
immunoprecipitated DNA using primers specific
for the human GAPDH CDS region, LDHA,
FOXO3a-promoter and FOXO3a-downstream regions.
Data are presented as enrichment of each
sample relative to total amount of input
chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • Histone 2-Hydroxyisobutyryltransferase Encoded by Afngg1 Is Involved in Pathogenicity and Aflatoxin Biosynthesis in Aspergillus flavus
    Year 2022
    Journal Toxins
    Authors Jing Wang, et al.
    Applications Unspecified
    Reactivity
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