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Anti-2-Hydroxyisobutyryllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-804
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: khib

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms khib
UniProt ID

/

Immunogen 2-Hydroxyisobutyrylated lysine peptides
MW (kDa) Multiple
Specificity With a mixture of high-quality rabbit- and mouse-derived anti-crotonyllysine antibodies cross-linked, this Anti-Phosphotyrosine Antibody Conjugated Agarose Beads present extraordinary capability to specifically capture peptides/proteins bearing crotonyllysine residues but do not cross-react with the peptides/proteins bearing structurally similar acetyllysine residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine crotonylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, etc. 2-Hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-2-Hydroxyisobutyryllysine Rabbit pAb (PTM-801) on different acylated peptides.
Peptides/Protein amount: 4 ng, 20 ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Acetylated peptide library. Lane 2: Butyrylated peptide library.
Lane 3: Crotonylated peptide library. Lane 4: Unmodified peptide library.
Lane 5: 2-Hydroxyisobutyrylated peptide library. Lane 6: 2-Hydroxybutyrylated peptide library.
Lane 7: β-Hydroxybutyrylated peptide library. Lane 8: 3-Hydroxyisobutyrylated peptide library.
Lane 9: 4-Hydroxybutyrylated peptide library.

Research Use

For research use only, not for use in diagnostic procedures.

References (27)
  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
    Year 2023
    Journal Advanced Science
    Authors Bin Chen, et al.
    Applications Unspecified
    Reactivity
  • Deciphering the Atlas of Post-Translational Modification in Sugarcane
    Year 2023
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Qibin Wu, et al.
    Applications Unspecified
    Reactivity
  • Qualitative lysine crotonylation and 2-hydroxyisobutyrylation analysis in the ovarian tissue proteome of piglets
    Year 2023
    Journal Frontiers in Cell and Developmental Biology
    Authors Diqi Yang, et al.
    Applications Unspecified
    Reactivity
  • Lysine Acylation Modification Landscape of Brucella abortus Proteome and its Virulent Proteins.
    Year 2022
    Journal Frontiers in Cell and Developmental Biology
    Authors Zhang X, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Proteomics Reveals the Role of Lysine 2-Hydroxyisobutyrylation Pathway Mediated by Tip60
    Year 2022
    Journal Oxidative Medicine and Cellular Longevity
    Authors Wang Ning, et al.
    Applications Unspecified
    Reactivity
  • Quantitative multiplexed proteomics analysis reveals reshaping of the lysine 2-hydroxyisobutyrylome in Fusarium graminearum by tebuconazole
    Year 2022
    Journal BMC GENOMICS
    Authors Zhao Yanxiang, et al.
    Applications Unspecified
    Reactivity
  • Functional interplay of histone lysine 2-hydroxyisobutyrylation and acetylation in Arabidopsis under dark-induced starvation
    Year 2021
    Journal NUCLEIC ACIDS RESEARCH
    Authors Zheng Lanlan, et al.
    Applications Unspecified
    Reactivity
  • TmcA functions as a lysine 2-hydroxyisobutyryltransferase to regulate transcription
    Year 2021
    Journal Nature Chemical Biology
    Authors Dong Hanyang, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Candida albicans | mSystems
    Year 2021
    Journal mSystems
    Authors Hailin Zheng, et al.
    Applications Unspecified
    Reactivity
  • Protein Modification Characteristics of the Malaria Parasite Plasmodium falciparum and the Infected Erythrocytes
    Year 2021
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Jianhua Wang, et al.
    Applications Unspecified
    Reactivity
  • Global Lysine Acetylation and 2-Hydroxyisobutyrylation Profiling Reveals the Metabolism Conversion Mechanism in Giardia lamblia
    Year 2021
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Wenhe Zhu, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation proteomics reveals protein modification alteration in the actin cytoskeleton pathway of oral squamous cell carcinoma
    Year 2021
    Journal Journal of Proteomics
    Authors Zeyu Zhang, et al.
    Applications Unspecified
    Reactivity
  • Salt stress downregulates 2-hydroxybutyrylation in Arabidopsis siliques
    Year 2021
    Journal Journal of Proteomics
    Authors Geriqiqige Hong, et al.
    Applications Unspecified
    Reactivity
  • Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy
    Year 2021
    Journal Clinical Proteomics
    Authors Huang Shaoying, et al.
    Applications Unspecified
    Reactivity
  • Co-occurrence of Protein Crotonylation and 2-Hydroxyisobutyrylation in the Proteome of End-Stage Renal Disease
    Year 2021
    Journal ACS Omega
    Authors Jingjing Dong, et al.
    Applications Unspecified
    Reactivity
  • The Landscape and Potential Regulatory Mechanism of Lysine 2-Hydroxyisobutyrylation of Protein in End-Stage Renal Disease
    Year 2021
    Journal NEPHRON
    Authors Fengping Zheng, et al.
    Applications Unspecified
    Reactivity
  • Proteome-Wide Analyses Reveal the Diverse Functions of Lysine 2-Hydroxyisobutyrylation in Oryza sativa
    Year 2020
    Journal Rice
    Authors Xue Chao, et al.
    Applications Unspecified
    Reactivity
  • Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry
    Year 2020
    Journal PARASITOLOGY RESEARCH
    Authors Nie Lan-Bi, et al.
    Applications Unspecified
    Reactivity
  • Genotyping, generation and proteomic profiling of the first human autosomal dominant osteopetrosis type II-specific induced pluripotent stem cells
    Year 2019
    Journal Stem Cell Research & Therapy
    Authors Ou Minglin, et al.
    Applications Unspecified
    Reactivity
  • Differential occurrence of lysine 2-hydroxyisobutyrylation in psoriasis skin lesions
    Year 2019
    Journal Journal of Proteomics
    Authors Huiyao Ge, et al.
    Applications Unspecified
    Reactivity
  • Systematic Identification of Lysine 2-hydroxyisobutyrylated Proteins in Proteus mirabilis*
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Hanyang Dong, et al.
    Applications Unspecified
    Reactivity
  • Global Analysis of Lysine 2-Hydroxyisobutyrylome upon SAHA Treatment and Its Relationship with Acetylation and Crotonylation
    Year 2018
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Quan Wu, et al.
    Applications Unspecified
    Reactivity
  • Landscape of the regulatory elements for lysine 2-hydroxyisobutyrylation pathway
    Year 2017
    Journal CELL RESEARCH
    Authors Huang He, et al.
    Applications Unspecified
    Reactivity
  • 2-Hydroxyisobutyrylation on histone H4K8 is regulated by glucose homeostasis in Saccharomyces cerevisiae
    Year 2017
    Journal PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    Authors Huang Jing, et al.
    Applications Unspecified
    Reactivity
  • Proteome-wide Analysis of Lysine 2-hydroxyisobutyrylation in Developing Rice (Oryza sativa) Seeds
    Year 2017
    Journal Scientific Reports
    Authors Meng Xiaoxi, et al.
    Applications Unspecified
    Reactivity
  • The rate of glycolysis quantitatively mediates specific histone acetylation sites
    Year 2015
    Journal Cancer & Metabolism
    Authors Cluntun, et al.
    Applications Unspecified
    Reactivity
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