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Anti-2-Hydroxyisobutyryllysine Mouse mAb
Catalog Number: PTM-802
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: Khib

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Khib
UniProt ID

/

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 4 ng, 20 ng, 100 ng
Exposure time: 60 seconds
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, N2a, BRL, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Research Use

For research use only, not for use in diagnostic procedures.

References (5)
  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
    Year 2023
    Journal CELL RESEARCH
    Authors Liao Long, et al.
    Applications Unspecified
    Reactivity
  • Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs
    Year 2023
    Journal Microbiome
    Authors Hu Jun, et al.
    Applications Unspecified
    Reactivity
  • Deciphering the Atlas of Post-Translational Modification in Sugarcane
    Year 2023
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Qibin Wu, et al.
    Applications Unspecified
    Reactivity
  • Integrated insights into the mechanisms underlying sepsis-induced myocardial depression using a quantitative global proteomic analysis
    Year 2022
    Journal Journal of Proteomics
    Authors Ni Yang, et al.
    Applications Unspecified
    Reactivity
  • Malonyl-proteome profiles of Staphylococcus aureus reveal lysine malonylation modification in enzymes involved in energy metabolism
    Year 2021
    Journal Proteome Science
    Authors Shi Yanan, et al.
    Applications Unspecified
    Reactivity
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