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Anti-2-Hydroxyisobutyryllysine Rabbit pAb
Catalog Number: PTM-801
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: All

Synonyms: Khib

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Khib
UniProt ID

/

Immunogen 2-Hydroxyisobutyrylated lysine peptides
MW (kDa) Multiple
Specificity Anti-2-Hydroxyisobuyrylated Rabbit pAb detects proteins with 2-hydroxyisobutyrylated lysine residues. This pan antibody recognizes 2-hydroxyisobutyrylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 All
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, and etc. Khib is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of Khib suggest that it is mechanistically and functionally different from histone lysine acetylation.

Cellular location

/

Images
Dot Blot

Peptide amount: 4 ng, 20 ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Acetylated peptide library.
Lane 2: Butyrylated peptide library.
Lane 3: Crotonylated peptide library.
Lane 4: Unmodified peptide library.
Lane 5: 2-Hydroxyisobutyrylated peptide library.
Lane 6: 2-Hydroxybutyrylated peptide library.
Lane 7: β-Hydroxybutyrylated peptide library.
Lane 8: 3-Hydroxyisobutyrylated peptide library.
Lane 9: 4-Hydroxybutyrylated peptide library.
WB

Lysates: HeLa, MEF, S2, S.cerevisiae
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

References (30)
  • The involvement of lysine glutarylation and mitochondria in spermatic movement deficiency induced by hexavalent chromium
    Year 2024
    Journal Heliyon
    Authors Yimin Cheng, et al.
    Applications Unspecified
    Reactivity
  • Glycometabolic reprogramming-induced XRCC1 lactylation confers therapeutic resistance in ALDH1A3-overexpressing glioblastoma
    Year 2024
    Journal Cell Metabolism
    Authors Guanzhang Li, et al.
    Applications Unspecified
    Reactivity
  • Astrocyte-derived lactate aggravates brain injury of ischemic stroke in mice by promoting the formation of protein lactylation
    Year 2024
    Journal Theranostics
    Authors Xiao-Yi Xiong, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation proteomics analyses reveal the regulatory mechanism of CaMYB61-CaAFR1 module in regulating stem development in Capsicum annuum L.
    Year 2024
    Journal PLANT JOURNAL
    Authors Qing Li, et al.
    Applications Unspecified
    Reactivity
  • The crotonylated and succinylated proteins of jujube involved in phytoplasma-stress responses
    Year 2024
    Journal BMC BIOLOGY
    Authors Liman Zhang, et al.
    Applications Unspecified
    Reactivity
  • Identification and Functional Analysis of Lysine 2-Hydroxyisobutyrylation in Cyanobacteria
    Year 2024
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Qiaoya Li, et al.
    Applications Unspecified
    Reactivity
  • Linking chromatin acylation mark-defined proteome and genome in living cells
    Year 2023
    Journal CELL
    Authors Fangfei Qin, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
    Year 2023
    Journal Advanced Science
    Authors Bin Chen, et al.
    Applications Unspecified
    Reactivity
  • Qualitative lysine crotonylation and 2-hydroxyisobutyrylation analysis in the ovarian tissue proteome of piglets
    Year 2023
    Journal Frontiers in Cell and Developmental Biology
    Authors Yang Diqi, et al.
    Applications Unspecified
    Reactivity
  • Hexachlorocyclohexane impairs human sperm motility by affecting lysine glutarylation and mitochondrial functions
    Year 2023
    Journal FOOD AND CHEMICAL TOXICOLOGY
    Authors Liu Yang, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Proteomics Reveals the Role of Lysine 2-Hydroxyisobutyrylation Pathway Mediated by Tip60
    Year 2022
    Journal Oxidative Medicine and Cellular Longevity
    Authors Ning Wang, et al.
    Applications Unspecified
    Reactivity
  • Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii
    Year 2022
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation orchestrates cell development and aflatoxin biosynthesis in Aspergillus flavus
    Year 2022
    Journal ENVIRONMENTAL MICROBIOLOGY
    Authors Yangyong Lv, et al.
    Applications Unspecified
    Reactivity
  • Functional interplay of histone lysine 2-hydroxyisobutyrylation and acetylation in Arabidopsis under dark-induced starvation
    Year 2021
    Journal NUCLEIC ACIDS RESEARCH
    Authors Zheng Lanlan, et al.
    Applications Unspecified
    Reactivity
  • Global Profiling of 2-hydroxyisobutyrylome in Common Wheat
    Year 2021
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Ning Zhang, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylated Proteins in Fusarium oxysporum
    Year 2021
    Journal Frontiers in Microbiology
    Authors Qian Hengwei, et al.
    Applications Unspecified
    Reactivity
  • Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis
    Year 2021
    Journal Scientific Reports
    Authors Perico Luca, et al.
    Applications Unspecified
    Reactivity
  • Salt stress downregulates 2-hydroxybutyrylation in Arabidopsis siliques
    Year 2021
    Journal Journal of Proteomics
    Authors Geriqiqige Hong, et al.
    Applications Unspecified
    Reactivity
  • Aspirin modulates 2-hydroxyisobutyrylation of ENO1K281 to attenuate the glycolysis and proliferation of hepatoma cells
    Year 2021
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Ying Yuan, et al.
    Applications Unspecified
    Reactivity
  • Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility
    Year 2020
    Journal HUMAN REPRODUCTION
    Authors Cheng Yi-min, et al.
    Applications Unspecified
    Reactivity
  • Proteome-Wide Analyses Reveal the Diverse Functions of Lysine 2-Hydroxyisobutyrylation in Oryza sativa
    Year 2020
    Journal Rice
    Authors Xue Chao, et al.
    Applications Unspecified
    Reactivity
  • Global Lysine Crotonylation and 2-Hydroxyisobutyrylation in Phenotypically Different Toxoplasma gondii Parasites *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
  • Elevated lysine crotonylation and succinylation in the brains of BTBR mice
    Year 2019
    Journal INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
    Authors Min Wang, et al.
    Applications Unspecified
    Reactivity
  • p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis
    Year 2018
    Journal MOLECULAR CELL
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • Landscape of the regulatory elements for lysine 2-hydroxyisobutyrylation pathway
    Year 2017
    Journal CELL RESEARCH
    Authors Huang He, et al.
    Applications Unspecified
    Reactivity
  • Proteome-wide Analysis of Lysine 2-hydroxyisobutyrylation in Developing Rice (Oryza sativa) Seeds
    Year 2017
    Journal Scientific Reports
    Authors Meng Xiaoxi, et al.
    Applications Unspecified
    Reactivity
  • Proteome-wide identification of lysine 2-hydroxyisobutyrylation reveals conserved and novel histone modifications in Physcomitrella patens
    Year 2017
    Journal Scientific Reports
    Authors Zhiming Yu, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark
    Year 2014
    Journal Nature Chemical Biology
    Authors Dai Lunzhi, et al.
    Applications Unspecified
    Reactivity
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