/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Anti-Acetyl-Histone H2A (Lys9) Rabbit pAb
Catalog Number: PTM-125
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H2AK9ac

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

  • View More Images(3)
    $ 340
    Anti-Acetyl-Histone H2A (Lys9) Mouse mAb
    Catalog #
    PTM-193
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(5)
    $ 330
    Recombinant Rabbit Monoclonal
    Anti-Acetyl-Histone H2A (Lys9) Rabbit mAb
    Catalog #
    PTM-194
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2AK9ac
UniProt ID

P04908

Immunogen Acetylated human histone H2A (Lys9) peptide
MW (kDa) 14
Specificity Anti-Acetyl-Histone H2A (Lys9) Rabbit pAb detects histone H2A only when it is acetylated at Lys9. This antibody has been shown to selectively recognize acetylated H2A peptide at Lys9, but not the structurally similar 2-hydroxyisobutyrylated, β-hydroxybutyrylated, succinylated H2A peptides at Lys5 or Lys9.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ChIP 4 μg per 5×106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided in the table below.

WB

Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 14 kDa
Observed band size: 14 kDa

ChIP

Cell type: HeLa cells + sodium butyrate (5 mM, 24 hours)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 4 μg, 12 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RAB20, GAPDH CDS, RPL30, LDHA, FOXO3a promoter, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
  • View More Images(4)
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Quantitative Proteomic Analysis Reveals the Sites Related to Acetylation and Mechanism of ACY-1215 in Acute Liver Failure Mice
    Year 2019
    Journal Frontiers in Pharmacology
    Authors Zhang Wen-bin, et al.
    Applications Unspecified
    Reactivity