/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Anti-Acetyl-Histone H2B (Lys120) Rabbit pAb
Catalog Number: PTM-111
$ 340

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H2BK120ac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2BK120ac
UniProt ID

P62807

Immunogen Acetylated human histone H2B (Lys120) peptide
MW (kDa) 14
Specificity Anti-Acetyl-Histone H2B (Lys120) Rabbit pAb detects histone H2B only when it is acetylated at Lys120. This antibody has been shown to selectively recognize acetylated H2B peptide at Lys120, but not the structurally similar succinylated peptide at Lys120 or unmodified peptide.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ChIP 4 μg per 5x106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided in the table below.

WB

Lysate: (-): HeLa cells; (+): HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 14 kDa
Observed band size: 14 kDa

ChIP

Cell type: HeLa cells, serum starvation for 12 hours, followed by incubation with 5 mM sodium butyrate for 24 hours.
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 4 μg, 12 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH promoter, GAPDH CDS, RPL30, FOXO3a promoter, and FOXO3a downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
  • View More Images(4)
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 435
    Anti-L-Lactyl-Histone H2B (Lys120) Rabbit pAb
    Catalog #
    PTM-1423
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(5)
    $ 375
    Recombinant Rabbit Monoclonal
    Anti-Ubiquityl-Histone H2B (Lys120) Rabbit mAb
    Catalog #
    PTM-1123RM
    Host
    Rabbit
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 420
    Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb
    Catalog #
    PTM-409
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B-related hepatocellular carcinoma
    Year 2021
    Journal Clinical and Translational Medicine
    Authors Xiaoqiang Chai, et al.
    Applications Unspecified
    Reactivity