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Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb
Catalog Number: PTM-116
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P ICC/IF ChIP

Reactivity: Human, Mouse, Rat, Pharbitis

Synonyms: H3K27ac

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

  • View More Images(7)
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb
    Catalog #
    PTM-116RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(4)
    $ 330
    Anti-Acetyl-Histone H3 (Lys27) Mouse mAb
    Catalog #
    PTM-160
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K27ac
UniProt ID

P68431

Immunogen Acetylated human histone H3 (Lys27) peptide
MW (kDa) 15
Specificity Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the crotonylated H3 peptide at Lys27 or unmodified peptide.
Product Usage Information
Applications Dilution Recommended Species
WB 1:400-1:2000 Human, Mouse, Rat, Pharbitis
IHC-P 1:50 - 1:200 Human
ICC/IF 1:50 - 1:200 Human
ChIP 1:100 - 1:500 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below:

WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate
(30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 17 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:400
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Pharbitis, N2a, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 17 kDa

IHC-P

Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer
HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Description: Color brown is the positive signal for
PTM-116

ChIP

Cell type: HeLa+Sodium butyrate (30mM, 4hr)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106
cells
Amount of Ab per IP: 1:500 (2 μl)
Beads type and amount per IP: 50 μl of
Protein A/G MagBeads
Description: The ChIP was performed with 1 μg of
normal rabbit IgG as a negative control. Real
time quantitative PCR was performed on
immunoprecipitated DNA using primers specific
for the human GAPDH Promoter, GAPDH CDS
region, RPL30 Exon 3 and MyoD1 Exon 1. Data
are presented as enrichment of each sample
relative to total amount of input chromatin at
each amplicon.

ChIP

Cell type: HeLa+Sodium butyrate (30mM, 4hr)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 1:500 (2 μl)
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Description: The ChIP was performed with 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH Promoter, GAPDH CDS region, RPL30 Exon 3 and MyoD1 Exon 1. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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    Journal Frontiers in Physiology
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