Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB IHC-P ICC/IF ChIP
Reactivity: Human, Mouse, Rat, Pharbitis
Synonyms: H3K27ac
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H3K27ac |
| UniProt ID | |
| Immunogen | Acetylated human histone H3 (Lys27) peptide |
| MW (kDa) | 15 |
| Specificity | Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the crotonylated H3 peptide at Lys27 or unmodified peptide. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:400-1:2000 | Human, Mouse, Rat, Pharbitis |
| IHC-P | 1:50 - 1:200 | Human |
| ICC/IF | 1:50 - 1:200 | Human |
| ChIP | 1:100 - 1:500 | Human |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.
Cellular location
Nucleus
| Dot Blot | |
|---|---|
|
Blocking buffer: 5% NFDM/TBST |
| WB | |
|---|---|
|
Blocking buffer: 5% NFDM/TBST |
|
Blocking buffer: 5% NFDM/TBST |
| IHC-P | |
|---|---|
|
Tissue: Human breast cancer |
| ChIP | |
|---|---|
|
Cell type: HeLa+Sodium butyrate (30mM, 4hr) |
| ChIP | |
|---|---|
|
|
Cell type: HeLa+Sodium butyrate (30mM, 4hr) |
Research Use
For research use only, not for use in diagnostic procedures.
