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Recombinant Rabbit Monoclonal
Anti-Acetyl-Histone H3 (Lys36) Rabbit mAb
Catalog Number: PTM-117RM
$ 330

Clone Number: PD-166-44

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF ChIP

Reactivity: Human, Mouse, Rat, Pig

Synonyms: H3K36ac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K36ac
UniProt ID

P68431

Immunogen Acetylated human histone H3 (Lys36) peptide
MW (kDa) 15
Specificity Anti-Acetyl-Histone H3 (Lys36) Rabbit mAb detects histone H3 only when it is acetylated at Lys36. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys36, but not the unmodified peptide.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:10000 Human, Mouse, Rat, Pig
IHC-P 1:100 - 1:500 Human, Mouse, Rat
ICC/IF 1:1000 - 1:2500 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-rabbit IgG H&L (HRP)
Peptide amount: 1 ng, 4 ng, 16 ng, 64ng
Exposure time: 10 s
The list of peptides is included in the table below:
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): MCF-7, (+): MCF-7+ Sodium Butyrate(50mM,24hr) + Trichostatin A(500ng-ml,4 hr)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C6, (+): C6 + Trichostatin A (500ng-ml, 4h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Mouse liver, 2: Rat brain, 3: Rat kideny, 4: Pig brain
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
IHC-P

Tissue: Human placenta
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-117RM

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-117RM

Tissue: Mouse cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-117RM

Tissue: Rat cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-117RM
ChIP

Cell type: HeLa treated with Sodium butyrate (5mM, 24hr)
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description:
The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real
time quantitative PCR was performed on immunoprecipitated DNA using primers
specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA CDS
region, FOXO3a-promoter, FOXO3a-downstream, RAB20 and TuBBP10. Data are
presented as enrichment of each sample relative to total amount of input chromatin at
each amplicon.
ICC/IF

Cell line: (A) HeLa, (B) HeLa + Sodium Butyrate (30 mM, 4hr)
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:2500
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (Red)
Description: Color green is the positive signal for PTM-117RM

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 340
    Anti-Acetyl-Histone H3 (Lys36) Rabbit pAb
    Catalog #
    PTM-117
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
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