中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb
Catalog Number: PTM-112RM
$ 325

Clone Number: PA-169-56

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ChIP CUT&Tag

Reactivity: Human, Mouse, Rat

Synonyms: H3K9ac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K9ac
UniProt ID

P68431

Immunogen Acetylated human histone H3 (Lys9) peptide
MW (kDa) 15
Specificity Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb detects histone H3 only when it is acetylated at Lys9. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys9, but not the structurally similar butyrylated, β-hydroxybutyrylated, or 2-hydroxyisobutyrylated peptide at Lys9 or Lys27. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys14, or Lys27.
Product Usage Information
Applications Dilution Recommended Species
WB 1:5000 - 1:10000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ChIP 6 μg/5x106 cells Human
CUT&Tag 1:20 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided in the table below.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 3 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: (-): NIH/3T3; (+): NIH/3T3 + sodium butyrate (500 ng/ml, 4 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 3 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysate: (-): C6; (+): C6 + sodium butyrate (500 ng/ml, 5 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human kidney
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-112RM.

Tissue: Human testis
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-112RM.

Tissue: Human spleen
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-112RM.

Tissue: Mouse stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-112RM.

Tissue: Rat stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-112RM.
ChIP

Cell type: HeLa+Sodium butyrate (5mM, 24hr)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Description:The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, LDHApromoter, LDHA CDS region, FOXO3a-promoter and RAB20. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.
CUT&Tag

Cell type: HeLa +Sodium butyrate (5 mM, 24 hr)
Quantity of cell: 5×104cells
Primary ab dilution: 1: 50
Primary ab incubation condition: 4℃ overnight
Secondary ab: Goat Anti-Rabbit IgG H&L pAb (PTM-6252)
Secondary ab dilution: 1: 700
Description: Genomic visualization of CUT&Tag sequencing results from Normal Rabbit IgG negative control and Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb (PTM-112RM). There are significant H3K9ac signal enrichments at the promoter regions of RBL30 and POP1/RIDA. DNA Libraries were sequenced with Illumina NovaSeq platform (PE 150) and sequencing depth of 3 M reads.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（6）
    $ 345
    Anti-Acetyl-Histone H3 (Lys9) Rabbit pAb
    Catalog #
    PTM-112
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 335
    Anti-Acetyl-Histone H3 (Lys9) Mouse mAb
    Catalog #
    PTM-156
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-5073
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-6628
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 280
    Recombinant
    Anti-SIRT1 Mouse mAb
    Catalog #
    PTM-5021
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 280
    Recombinant
    Anti-SIRT2 Mouse mAb
    Catalog #
    PTM-6043
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-SIRT6 Rabbit mAb
    Catalog #
    PTM-6658
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-ELP3 Rabbit mAb
    Catalog #
    PTM-5980
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-CBP Rabbit mAb
    Catalog #
    PTM-5281
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-PCAF Rabbit mAb
    Catalog #
    PTM-5711
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 280
    Recombinant
    Anti-GCN5 Mouse mAb
    Catalog #
    PTM-5115
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（10）
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Acetyllysine Rabbit mAb
    Catalog #
    PTM-105RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 1,800
    Recombinant Rabbit Monoclonal
    Anti-Acetyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-104
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 330
    Anti-Trimethyl-Histone H3 (Lys9) Rabbit pAb
    Catalog #
    PTM-616
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-β-Hydroxybutyryl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-1250RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-1419RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 420
    Anti-Butyryl-Histone H3 (Lys9) Mouse mAb
    Catalog #
    PTM-312
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 330
    Recombinant Rabbit Monoclonal
    Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-615RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（10）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Crotonyl-histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-516RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Erchen Decoction alleviates obesity-related hepatic steatosis via modulating gut microbiota-drived butyric acid contents and promoting fatty acid β-oxidation
    Year 2023
    Journal JOURNAL OF ETHNOPHARMACOLOGY
    Authors Ling Zhang, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.