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Recombinant Rabbit Monoclonal
Anti-Acetyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-104
$ 1,800

Clone Number: /

Host: / Clonality: /

Applications: IAP

Reactivity: All

Synonyms: Kac

Product Size
500μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity With a mixture of high-quality rabbit- and mouse-derived anti-acetyllysine antibodies cross-linked, this Anti-Acetyllysine Antibody Conjugated Agarose Beads present extraordinary capability to specifically capture peptides/proteins bearing acetyllysine residues but do not cross-react with the peptides/proteins bearing structurally similar propionyllysine or butyryllysine residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine acetylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene activities, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. The affinity purification with Anti-Acetyllysine Antibody Conjugated Agarose Beads followed by MS/MS identification is viewed as the most preferable and efficient method for systematical screening of lysine acetylated proteins.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Acetyllysine Rabbit pAb (PTM-105) on different acylation proteins.
Peptide/Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided below.
Lane 1: Acetylated peptide library. Lane 2: Acetylated BSA. Lane 3: Propionylated BSA
Lane 4: Butyrylated BSA. Lane 5: BSA

Research Use

For research use only, not for use in diagnostic procedures.

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References (156)
  • Hepatic steatosis is associated with dysregulated cholesterol metabolism and altered protein acetylation dynamics in chickens
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    Authors Guo Xiaoli, et al.
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  • Proteomics and Lysine Acetylation Modification Reveal the Responses of Pakchoi (Brassica rapa L. ssp. chinensis) to Oxybenzone Stress
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    Reactivity
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    Reactivity
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    Year 2021
    Journal Scientific Reports
    Authors Jiang Na, et al.
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    Reactivity
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    Year 2021
    Journal Frontiers in Neurology
    Authors Ya-Wen Xu, et al.
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    Year 2021
    Journal Clinical Proteomics
    Authors Wang Bo, et al.
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    Year 2021
    Journal PROTEOMICS
    Authors Shuai Kong, et al.
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    Year 2021
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  • The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum
    Year 2018
    Journal NEW PHYTOLOGIST
    Authors Yanni Yin, et al.
    Applications Unspecified
    Reactivity
  • Lysine acetylation of DosR regulates the hypoxia response of Mycobacterium tuberculosis
    Year 2018
    Journal Emerging Microbes & Infections
    Authors Hua Yang, et al.
    Applications Unspecified
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  • Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves
    Year 2018
    Journal PLANT CELL AND ENVIRONMENT
    Authors Heng Zhou, et al.
    Applications Unspecified
    Reactivity
  • Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum*
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Jun-Yu Xu, et al.
    Applications Unspecified
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  • Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum*
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zhuo Chen, et al.
    Applications Unspecified
    Reactivity
  • Establishment of Dimethyl Labeling-based Quantitative Acetylproteomics in Arabidopsis *
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Shichang Liu, et al.
    Applications Unspecified
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  • Acetylome in Human Fibroblasts From Parkinson's Disease Patients
    Year 2018
    Journal Frontiers in Cellular Neuroscience
    Authors Yakhine-Diop Sokhna M. S, et al.
    Applications Unspecified
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  • Comprehensive Proteomic Analysis of Lysine Acetylation in the Foodborne Pathogen Trichinella spiralis
    Year 2018
    Journal Frontiers in Microbiology
    Authors Yang Yong, et al.
    Applications Unspecified
    Reactivity
  • Acetyl-CoA from inflammation-induced fatty acids oxidation promotes hepatic malate-aspartate shuttle activity and glycolysis
    Year 2018
    Journal AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
    Authors Tongxin Wang, et al.
    Applications Unspecified
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  • Quantitative Dynamics of Proteome, Acetylome, and Succinylome during Stem-Cell Differentiation into Hepatocyte-like Cells
    Year 2018
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Zekun Liu, et al.
    Applications Unspecified
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  • The First Whole-Cell Proteome- and Lysine-Acetylome-Based Comparison between Trichophyton rubrum Conidial and Mycelial Stages
    Year 2018
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Xingye Xu, et al.
    Applications Unspecified
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  • Comprehensive Analysis of Lysine Acetylome Reveals a Site-Specific Pattern in Rapamycin-Induced Autophagy
    Year 2018
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Zhuo Zhou, et al.
    Applications Unspecified
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  • Comprehensive proteome analyses of lysine acetylation in tea leaves by sensing nitrogen nutrition
    Year 2018
    Journal BMC GENOMICS
    Authors Jiang Jutang, et al.
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  • Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection
    Year 2018
    Journal BMC GENOMICS
    Authors Li Hongran, et al.
    Applications Unspecified
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  • First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents
    Year 2018
    Journal BMC GENOMICS
    Authors Hui Min, et al.
    Applications Unspecified
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  • Mass spectrometry and DigiWest technology emphasize protein acetylation profile from Quisinostat-treated HuT78 CTCL cell line
    Year 2018
    Journal Journal of Proteomics
    Authors Bruno Méhul, et al.
    Applications Unspecified
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  • The lysine acetylome of the nematocidal bacterium Bacillus nematocida and impact of nematode on the acetylome
    Year 2018
    Journal Journal of Proteomics
    Authors Xin-Li Sun, et al.
    Applications Unspecified
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  • Comprehensive analysis of the lysine acetylome and its potential regulatory roles in the virulence of Streptococcus pneumoniae
    Year 2018
    Journal Journal of Proteomics
    Authors Yu-Tong Liu, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (Bombyx mori) Cells
    Year 2018
    Journal PROTEOMICS
    Authors Dongbing Hu, et al.
    Applications Unspecified
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  • Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues
    Year 2018
    Journal Clinical Proteomics
    Authors Yi Jinling, et al.
    Applications Unspecified
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  • Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
    Year 2018
    Journal Frontiers in Physiology
    Authors Sun Lina, et al.
    Applications Unspecified
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  • Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Oryza sativa L.)
    Year 2018
    Journal MOLECULES
    Authors Zhiyong Li, et al.
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  • First acetyl-proteome profiling of Salmonella Typhimurium revealed involvement of lysine acetylation in drug resistance
    Year 2018
    Journal VETERINARY MICROBIOLOGY
    Authors Lin Li, et al.
    Applications Unspecified
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  • Global analysis of histone lysine acetylation and proteomic changes in EC109 cells treated with the histone deacetylase inhibitor FK228
    Year 2018
    Journal Oncology Letters
    Authors Zhiwen Pan, et al.
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  • Lysine Acetylome Analysis Reveals Photosystem II Manganese-stabilizing Protein Acetylation is Involved in Negative Regulation of Oxygen Evolution in Model Cyanobacterium Synechococcus sp. PCC 7002 *
    Year 2017
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zhuo Chen, et al.
    Applications Unspecified
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  • Proteomic analysis of lysine acetylation provides strong evidence for involvement of acetylated proteins in plant meiosis and tapetum function
    Year 2017
    Journal PLANT JOURNAL
    Authors Xiaojing Li, et al.
    Applications Unspecified
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  • ING5 differentially regulates protein lysine acetylation and promotes p300 autoacetylation
    Year 2017
    Journal Oncotarget
    Authors Tao Zhang, et al.
    Applications Unspecified
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  • Proteome and Acetyl-Proteome Profiling of Camellia sinensis cv. ‘Anji Baicha’ during Periodic Albinism Reveals Alterations in Photosynthetic and Secondary Metabolite Biosynthetic Pathways
    Year 2017
    Journal Frontiers in Plant Science
    Authors Xu Yan-Xia, et al.
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  • Systematic Analysis of Lysine Acetylation in the Halophilic Archaeon Haloferax mediterranei
    Year 2017
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Jingfang Liu, et al.
    Applications Unspecified
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  • Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
    Year 2017
    Journal Scientific Reports
    Authors Zheng Xiangfeng, et al.
    Applications Unspecified
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  • Proteome and Acetylome Analysis Identifies Novel Pathways and Targets Regulated by Perifosine in Neuroblastoma
    Year 2017
    Journal Scientific Reports
    Authors Gu Xiao, et al.
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  • Global Insight into Lysine Acetylation Events and Their Links to Biological Aspects in Beauveria bassiana, a Fungal Insect Pathogen
    Year 2017
    Journal Scientific Reports
    Authors Wang Zhi-Kang, et al.
    Applications Unspecified
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  • Large-scale identification of lysine acetylated proteins in vegetative hyphae of the rice blast fungus
    Year 2017
    Journal Scientific Reports
    Authors Sun Xiaomei, et al.
    Applications Unspecified
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  • Comparative Analysis of Proteome-Wide Lysine Acetylation in Juvenile and Adult Schistosoma japonicum
    Year 2017
    Journal Frontiers in Microbiology
    Authors Li Qing, et al.
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  • A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.)
    Year 2017
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Yifeng Wang, et al.
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  • Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus
    Year 2017
    Journal PLoS One
    Authors Yangyong Lv, et al.
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  • Proteome-wide lysine acetylation identification in developing rice (Oryza sativa) seeds and protein co-modification by acetylation, succinylation, ubiquitination, and phosphorylation
    Year 2017
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
    Authors Xiaoxi Meng, et al.
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  • Quantitative proteomics analysis reveals alterations of lysine acetylation in mouse testis in response to heat shock and X-ray exposure
    Year 2017
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
    Authors Chengmei Xie, et al.
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  • Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica
    Year 2017
    Journal AMB Express
    Authors Wang Guangyuan, et al.
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  • Acetylome analysis reveals the involvement of lysine acetylation in diverse biological processes in Phytophthora sojae
    Year 2016
    Journal Scientific Reports
    Authors Li Delong, et al.
    Applications Unspecified
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  • Proteome-wide analysis of lysine acetylation in the plant pathogen Botrytis cinerea
    Year 2016
    Journal Scientific Reports
    Authors Lv Binna, et al.
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  • Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
    Year 2016
    Journal Scientific Reports
    Authors Zhu Xiaoyu, et al.
    Applications Unspecified
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  • First Comprehensive Proteome Analyses of Lysine Acetylation and Succinylation in Seedling Leaves of Brachypodium distachyon L.
    Year 2016
    Journal Scientific Reports
    Authors Zhen Shoumin, et al.
    Applications Unspecified
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  • Acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in Bacillus amyloliquefaciens
    Year 2016
    Journal Scientific Reports
    Authors Liu Lin, et al.
    Applications Unspecified
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  • Comprehensive profiling of lysine acetylproteome analysis reveals diverse functions of lysine acetylation in common wheat
    Year 2016
    Journal Scientific Reports
    Authors Zhang Yumei, et al.
    Applications Unspecified
    Reactivity
  • Crosstalk among proteome, acetylome and succinylome in colon cancer HCT116 cell treated with sodium dichloroacetate
    Year 2016
    Journal Scientific Reports
    Authors Zhu Danxi, et al.
    Applications Unspecified
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  • Lysine Acetylation and Succinylation in HeLa Cells and their Essential Roles in Response to UV-induced Stress
    Year 2016
    Journal Scientific Reports
    Authors Xu Hong, et al.
    Applications Unspecified
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  • Crosstalk among the proteome, lysine phosphorylation, and acetylation in romidepsin-treated colon cancer cells
    Year 2016
    Journal Oncotarget
    Authors Tian-Yun Wang, et al.
    Applications Unspecified
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  • Global Lysine Acetylome Analysis of Desiccated Somatic Embryos of Picea asperata
    Year 2016
    Journal Frontiers in Plant Science
    Authors Xia Yan, et al.
    Applications Unspecified
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  • Systematic Analysis of the Lysine Acetylome in Candida albicans
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Xiaowei Zhou, et al.
    Applications Unspecified
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  • Identification of Lysine Acetylation in Mycobacterium abscessus Using LC–MS/MS after Immunoprecipitation
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Jintao Guo, et al.
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  • Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Dongli He, et al.
    Applications Unspecified
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  • Global profiling of lysine acetylation in human histoplasmosis pathogen Histoplasma capsulatum
    Year 2016
    Journal INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
    Authors Longxiang Xie, et al.
    Applications Unspecified
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  • Systematic analysis of the lysine acetylome in Fusarium graminearum
    Year 2016
    Journal BMC GENOMICS
    Authors Zhou Shanyue, et al.
    Applications Unspecified
    Reactivity
  • Global-scale profiling of differential expressed lysine acetylated proteins in colorectal cancer tumors and paired liver metastases
    Year 2016
    Journal Journal of Proteomics
    Authors Zhanlong Shen, et al.
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  • Systematic analysis of the lysine acetylome of the pathogenic bacterium Spiroplasma eriocheiris reveals acetylated proteins related to metabolism and helical structure
    Year 2016
    Journal Journal of Proteomics
    Authors Qingguo Meng, et al.
    Applications Unspecified
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  • A comprehensive catalog of the lysine-acetylation targets in rice (Oryza sativa) based on proteomic analyses
    Year 2016
    Journal Journal of Proteomics
    Authors Yehui Xiong, et al.
    Applications Unspecified
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  • Global proteomic analysis of lysine acetylation in zebrafish (Danio rerio) embryos
    Year 2016
    Journal ELECTROPHORESIS
    Authors Oh Kwang Kwon, et al.
    Applications Unspecified
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  • Data for global lysine-acetylation analysis in rice (Oryza sativa)
    Year 2016
    Journal Data in Brief
    Authors Yehui Xiong, et al.
    Applications Unspecified
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  • Functional Characterization of Sirtuin-like Protein in Mycobacterium smegmatis
    Year 2015
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Lixiao Gu, et al.
    Applications Unspecified
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  • Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp. PCC 6803
    Year 2015
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Ran Mo, et al.
    Applications Unspecified
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  • Global Analysis of Protein Lysine Succinylation Profiles and Their Overlap with Lysine Acetylation in the Marine Bacterium Vibrio parahemolyticus
    Year 2015
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Jianyi Pan, et al.
    Applications Unspecified
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  • Global analysis of lysine acetylation in strawberry leaves
    Year 2015
    Journal Frontiers in Plant Science
    Authors Fang Xianping, et al.
    Applications Unspecified
    Reactivity
  • Proteomic profiling of lysine acetylation in Pseudomonas aeruginosa reveals the diversity of acetylated proteins
    Year 2015
    Journal PROTEOMICS
    Authors Tassadit Ouidir, et al.
    Applications Unspecified
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  • Persistent human Borna disease virus infection modifies the acetylome of human oligodendroglia cells towards higher energy and transporter levels
    Year 2015
    Journal VIROLOGY
    Authors Xia Liu, et al.
    Applications Unspecified
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  • Global proteomic analysis of protein acetylation affecting metabolic regulation in Daphnia pulex
    Year 2015
    Journal BIOCHIMIE
    Authors Oh Kwang Kwon, et al.
    Applications Unspecified
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  • Systematic Analysis of the Lysine Acetylome in Vibrio parahemolyticus
    Year 2014
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Jianyi Pan, et al.
    Applications Unspecified
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  • Proteome-wide lysine acetylation profiling of the human pathogen Mycobacterium tuberculosis
    Year 2014
    Journal INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
    Authors Longxiang Xie, et al.
    Applications Unspecified
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  • Unexpected extensive lysine acetylation in the trump-card antibiotic producer Streptomyces roseosporus revealed by proteome-wide profiling
    Year 2014
    Journal Journal of Proteomics
    Authors Guojian Liao, et al.
    Applications Unspecified
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  • Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells
    Year 2014
    Journal VIROLOGY
    Authors Xia Liu, et al.
    Applications Unspecified
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  • Comprehensive profiling of lysine acetylome in Staphylococcus aureus
    Year 2014
    Journal Science China-Chemistry
    Authors Zhang Yi, et al.
    Applications Unspecified
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  • SIRT4 Coordinates the Balance between Lipid Synthesis and Catabolism by Repressing Malonyl CoA Decarboxylase
    Year 2013
    Journal MOLECULAR CELL
    Authors Gaëlle Laurent, et al.
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  • Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli*
    Year 2013
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Gozde Colak, et al.
    Applications Unspecified
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  • SAHA Treatment Reveals the Link between Histone Lysine Acetylation and Proteome in Nonsmall Cell Lung Cancer A549 Cells
    Year 2013
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Quan Wu, et al.
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  • Quantitative Acetylome Analysis Reveals the Roles of SIRT1 in Regulating Diverse Substrates and Cellular Pathways *
    Year 2012
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yue Chen, et al.
    Applications Unspecified
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