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Anti-Acetyllysine Mouse mAb
Catalog Number: PTM-102
$ 330

Clone Number: Kac-11

Host: Mouse Clonality: Monoclonal

Applications： WB ICC/IF

Reactivity: All

Synonyms: Kac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Acetyllysine Mouse mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 All
ICC/IF 1:50 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.

Cellular location

/

Images
Dot Blot

Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Acetylated BSA. Lane 2: Propionylated BSA. Lane 3: Butyrylated BSA.
Lane 4: Unmodified BSA.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: (-) HepG2 cells; (+) HepG2 cells treated with 5 mM sodium butyrate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells treated with 5 mM sodium butyrate for 24 hours
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-102.

Research Use

For research use only, not for use in diagnostic procedures.

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References (28)
  • MYST1-Mediated Lysine Acetylation Stabilizes KLF4 to Promote Intimal Hyperplasia
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    Journal FASEB JOURNAL
    Authors Shizhi Wang, et al.
    Applications Unspecified
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  • CD44 is a macrophage receptor for TcdB from Clostridioides difficile that via its lysine-158 succinylation contributes to inflammation
    Year 2025
    Journal Gut Microbes
    Authors Zhuo Chen, et al.
    Applications Unspecified
    Reactivity
  • Reversible acetylation of ribosomal protein S1 serves as a smart switch for Salmonella to rapidly adapt to host stress
    Year 2025
    Journal NUCLEIC ACIDS RESEARCH
    Authors Shen Yi-Lin, et al.
    Applications Unspecified
    Reactivity
  • Genome-wide identification of glutathione S-transferase (GST) gene family and its regulating characteristics responding to sperm capacitation in Eriocheir sinensis
    Year 2025
    Journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Authors Xiaodong Cui, et al.
    Applications Unspecified
    Reactivity
  • Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm
    Year 2025
    Journal Nature Cardiovascular Research
    Authors Zhang Jingjing, et al.
    Applications Unspecified
    Reactivity
  • Importance of benzoyltransferase GcnE and lysine benzoylation of alcohol dehydrogenase AdhB in pathogenesis and aflatoxin production in Aspergillus flavus
    Year 2024
    Journal mBio
    Authors Xuan Chen, et al.
    Applications Unspecified
    Reactivity
  • Increased DNMT1 acetylation leads to global DNA methylation suppression in follicular granulosa cells during reproductive aging in mammals
    Year 2024
    Journal BMC GENOMICS
    Authors Zhao Shunran, et al.
    Applications Unspecified
    Reactivity
  • Nucleolin Malonylation as a Nuclear-Cytosol Signal Exchange Mechanism to Drive Cell Proliferation in Hepatocarcinoma by Enhancing AKT Translation
    Year 2024
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Liang Sun, et al.
    Applications Unspecified
    Reactivity
  • A network of acetyl phosphate-dependent modification modulates c-di-AMP homeostasis in Actinobacteria
    Year 2024
    Journal mBio
    Authors Yu Fu, et al.
    Applications Unspecified
    Reactivity
  • Sirtulin–Ypk1 regulation axis governs the TOR signaling pathway and fungal pathogenicity in Cryptococcus neoformans
    Year 2024
    Journal Microbiology Spectrum
    Authors Zhenghua Chai, et al.
    Applications Unspecified
    Reactivity
  • A proximity labeling-based orthogonal trap strategy identifies HDAC8 promotes cell motility by modulating cortactin acetylation
    Year 2024
    Journal Cell Chemical Biology
    Authors Yepei Huang, et al.
    Applications Unspecified
    Reactivity
  • A meet-up of acetyl phosphate and c-di-GMP modulates BldD activity for development and antibiotic production
    Year 2023
    Journal NUCLEIC ACIDS RESEARCH
    Authors Fu Yu, et al.
    Applications Unspecified
    Reactivity
  • BmCBP Catalyzes the Acetylation of BmApoLp-II Protein and Regulates Its Stability in Silkworm, Bombyx mori
    Year 2023
    Journal Insects
    Authors Yanmei Chen, et al.
    Applications Unspecified
    Reactivity
  • IL-1β-associated NNT acetylation orchestrates iron-sulfur cluster maintenance and cancer immunotherapy resistance
    Year 2023
    Journal MOLECULAR CELL
    Authors Yi Han, et al.
    Applications Unspecified
    Reactivity
  • ROS-stimulated Protein Lysine Acetylation Is Required for Crown Root Development in Rice
    Year 2022
    Journal Journal of Advanced Research
    Authors Qiutao Xu, et al.
    Applications Unspecified
    Reactivity
  • TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis
    Year 2022
    Journal EMBO JOURNAL
    Authors Qichao Huang, et al.
    Applications Unspecified
    Reactivity
  • HDAC inhibitors improve CRISPR/Cas9 mediated prime editing and base editing
    Year 2022
    Journal Molecular Therapy-Nucleic Acids
    Authors Nan Liu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum
    Year 2022
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Fangjiao Lv, et al.
    Applications Unspecified
    Reactivity
  • Selective recruitment of stress-responsive mRNAs to ribosomes for translation by acetylated protein S1 during nutrient stress in Escherichia coli
    Year 2022
    Journal Communications Biology
    Authors Zhang Bai-Qing, et al.
    Applications Unspecified
    Reactivity
  • Overexpression of Tfap2a in Mouse Oocytes Impaired Spindle and Chromosome Organization
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Juan Lin, et al.
    Applications Unspecified
    Reactivity
  • Cold Atmospheric Plasma Conveys Selectivity Against Hepatocellular Carcinoma Cells via Triggering EGFR(Tyr1068)-Mediated Autophagy
    Year 2022
    Journal Frontiers in Oncology
    Authors Danjun Wang, et al.
    Applications Unspecified
    Reactivity
  • ROS-stimulated protein lysine acetylation is required for crown root development in rice
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    Authors Qiutao Xu, et al.
    Applications Unspecified
    Reactivity
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    Authors Nan Liu, et al.
    Applications Unspecified
    Reactivity
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    Year 2021
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    Authors Wang Meng, et al.
    Applications Unspecified
    Reactivity
  • The human brain acetylome reveals that decreased acetylation of mitochondrial proteins associates with Alzheimer's disease
    Year 2021
    Journal JOURNAL OF NEUROCHEMISTRY
    Authors Lidan Sun, et al.
    Applications Unspecified
    Reactivity
  • Starvation promotes histone lysine butyrylation in the liver of male but not female mice
    Year 2020
    Journal GENE
    Authors Afsaneh Goudarzi, et al.
    Applications Unspecified
    Reactivity
  • Protein kinase Cα mediates recovery of renal and mitochondrial functions following acute injury
    Year 2019
    Journal FEBS Journal
    Authors Grazyna Nowak, et al.
    Applications Unspecified
    Reactivity
  • Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed
    Year 2016
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    Authors Dongli He, et al.
    Applications Unspecified
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