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Recombinant Rabbit Monoclonal
Anti-Acetyllysine Rabbit mAb
Catalog Number: PTM-105RM
$ 320

Clone Number: PA-070-53

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P IP ChIP

Reactivity: All

Synonyms: Kac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Acetyllysine Rabbit mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 All
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ChIP 6 μg per 5x106 cells All
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.

Cellular location

/

Images
Dot Blot

Peptide amount: 4 ng, 20ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Acetylated peptide library. Lane 2: Crotonylated peptide library.
Lane 3: Butyrylated peptide library. Lane 4: 2-Hydroxyisobutyrylated peptide library.
Lane 5: β-Hydroxybutyrylated peptide library. Lane 6: Unmodified peptide library.
WB

Lysate: Hela cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab: Lane 1, PTM-105RM pre-adsorbed with 3 μM synthetic acetylated lysine peptides; Lane 2, PTM-105RM pre-adsorbed with 3μM unmodified peptides.
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: 1: Hela; 2: NIH-3T3; 3: BRL; 4: Mouse liver; 5: Mouse kidney
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: (-): HeLa cells, (+): HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60+ seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: (-): HepG2 cells, (+): HepG cells treated with 5 mM sodium butyrate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60+ seconds
Predicted band size: Multiple
Observed band size: Multiple
IHC-P

Tissue: Human liver
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105RM.

Tissue: Mouse cerebrum
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105RM.

Tissue: Rat liver
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105RM.

Research Use

For research use only, not for use in diagnostic procedures.

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References (33)
  • SIRT6 knockdown alleviates keratinocyte hyperproliferation and inflammation in psoriasis via modulating acetylation of FOXO1
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    Year 2024
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    Authors Junfeng Zhen, et al.
    Applications Unspecified
    Reactivity
  • Interplay between acetylation and ubiquitination controls PSAT1 protein stability in lung adenocarcinoma
    Year 2024
    Journal Communications Biology
    Authors Liu Yuhan, et al.
    Applications Unspecified
    Reactivity
  • Nicotinamide enhances Treg differentiation by promoting Foxp3 acetylation in immune thrombocytopenia
    Year 2024
    Journal BRITISH JOURNAL OF HAEMATOLOGY
    Authors Ju Li, et al.
    Applications Unspecified
    Reactivity
  • Rheb1 deficiency elicits mitochondrial dysfunction and accelerates podocyte senescence through promoting Atp5f1c acetylation
    Year 2024
    Journal CELLULAR SIGNALLING
    Authors Qingmiao Lu, et al.
    Applications Unspecified
    Reactivity
  • Inhibition of HDAC8 mitigates AKI by reducing DNA damage and promoting homologous recombination repair
    Year 2024
    Journal JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
    Authors Yanjin Wang, et al.
    Applications Unspecified
    Reactivity
  • Calotropin attenuates ischemic heart failure after myocardial infarction by modulating SIRT1/FOXD3/SERCA2a pathway
    Year 2024
    Journal BIOMEDICINE & PHARMACOTHERAPY
    Authors Zijing Chen, et al.
    Applications Unspecified
    Reactivity
  • MOF-mediated acetylation of CDK9 promotes global transcription by modulating P-TEFb complex formation
    Year 2024
    Journal FEBS Journal
    Authors Wenqi Chen, et al.
    Applications Unspecified
    Reactivity
  • Hexokinase HK3-mediated O-GlcNAcylation of EP300: a key regulator of PD-L1 expression and immune evasion in ccRCC
    Year 2024
    Journal Cell Death & Disease
    Authors Zhang Wei, et al.
    Applications Unspecified
    Reactivity
  • Decrotonylation of cGAS K254 prompts homologous recombination repair by blocking its DNA binding and releasing PARP1
    Year 2024
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Hejiang Guo, et al.
    Applications Unspecified
    Reactivity
  • Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis
    Year 2024
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    Authors Li Yinzheng, et al.
    Applications Unspecified
    Reactivity
  • Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression
    Year 2024
    Journal CANCER LETTERS
    Authors Jiazhou Liu, et al.
    Applications Unspecified
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  • Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF
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    Authors Pan Botao, et al.
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  • Histone H3K18 and Ezrin Lactylation Promote Renal Dysfunction in Sepsis-Associated Acute Kidney Injury
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    Authors Jiao Qiao, et al.
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    Authors Fan Song, et al.
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  • EPB41L4A-AS1 is required to maintain basal autophagy to modulates Aβ clearance
    Year 2024
    Journal npj Aging
    Authors Wang Ziqiang, et al.
    Applications Unspecified
    Reactivity
  • SIRT1 maintains bone homeostasis by regulating osteoblast glycolysis through GOT1
    Year 2024
    Journal CELLULAR AND MOLECULAR LIFE SCIENCES
    Authors Jin Xinxin, et al.
    Applications Unspecified
    Reactivity
  • NDUFA9 and its crotonylation modification promote browning of white adipocytes by activating mitochondrial function in mice
    Year 2024
    Journal INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
    Authors Yuexia Liu, et al.
    Applications Unspecified
    Reactivity
  • Dihydrolipoyl dehydrogenase promotes white adipocytes browning by activating the RAS/ERK pathway and undergoing crotonylation modification
    Year 2024
    Journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Authors Yuexia Liu, et al.
    Applications Unspecified
    Reactivity
  • Histidine re-sensitizes pediatric acute lymphoblastic leukemia to 6-mercaptopurine through tetrahydrofolate consumption and SIRT5-mediated desuccinylation
    Year 2024
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    Authors Dong Na, et al.
    Applications Unspecified
    Reactivity
  • STEAP3 promotes colon cancer cell proliferation and migration via regulating histone acetylation
    Year 2024
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    Authors Lv Jinjuan, et al.
    Applications Unspecified
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  • Deacetylase Sirtuin 1 mitigates type I IFN- and type II IFN-induced signaling and antiviral immunity
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    Authors Shuang-Shuang Yu, et al.
    Applications Unspecified
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    Year 2023
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    Authors Tingting Wu, et al.
    Applications Unspecified
    Reactivity
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    Applications Unspecified
    Reactivity
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    Year 2023
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    Authors Song Fan, et al.
    Applications Unspecified
    Reactivity
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    Year 2023
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    Authors Qifan Hu, et al.
    Applications Unspecified
    Reactivity
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    Reactivity
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    Applications Unspecified
    Reactivity
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    Applications Unspecified
    Reactivity
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    Applications Unspecified
    Reactivity
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    Year 2023
    Journal Cell Death & Disease
    Authors Li Ranran, et al.
    Applications Unspecified
    Reactivity
  • Adenosine triphosphate can act as a determinant of lysine acetylation of non-native and native substrates
    Year 2022
    Journal MICROBIOLOGICAL RESEARCH
    Authors Jia-Peng Zhou, et al.
    Applications Unspecified
    Reactivity
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