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Anti-Acetyllysine Rabbit pAb
Catalog Number: PTM-105
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB IHC-P IP ChIP

Reactivity: All

Synonyms: Kac

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

Alternative Products
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    Anti-Acetyllysine Mouse mAb
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    Anti-Acetyllysine Rabbit pAb (BSA and Azide Free)
    Catalog #
    PTM-132
    Host
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    Clonality
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    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Acetyllysine Rabbit pAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:50 - 1:1000 Human, Mouse, Rat
IP 1:25 - 1:500 Human, Mouse
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.

Cellular location

/

Images
Dot Blot

Peptide/Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided below.
Lane 1: Acetylated peptide library. Lane 2: Acetylated BSA. Lane 3: Propionylated BSA.
Lane 4: Butyrylated BSA. Lane 5: BSA.
WB

Lysate: HeLa cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab: Lane A, PTM-105 pre-adsorbed with BSA; Lane B, PTM-105 pre-adsorbed with acetylated BSA
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple
IHC-P

Tissue: Human colon
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Human liver
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Mouse stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Rat stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.
IP

IP of Hela + sodium butyrate (30 mM, 4 hours) cells extracts
IP Ab incubation condition: PTM-105, 4°C overnight, 1:25, 1:100, 1:300 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: (Left) PTM-152, Anti-H2BK5ac Mouse mAb; (Right) PTM-157, Anti-H3K14ac Mouse mAb; 2 hours at room temperature, 1:1000 dilution
Secondary Ab: Goat Anti-Mouse IgG pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 14 kDa / 15 kDa
Observed band size: 14 kDa / 17 kDa

IP of Mouse liver cells extracts
IP Ab incubation condition: PTM-105, 4°C overnight, 1:25, 1:100 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: (Left) PTM-164, Anti-H4K8ac Mouse mAb; (Right) PTM-157, Anti-H3K14ac Mouse mAb; 2 h at room temperature, 1:1000 dilution
Secondary Ab: Goat Anti-Mouse IgG pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 11 kDa / 15 kDa
Observed band size: 11 kDa / 17 kDa
ChIP

Sample: HeLa cells, serum starvation for 12 hours, followed by incubation with 5 mM sodium butyrate for 24 hours.
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30, LDHA promoter, LDHA, FOXO3a promoter, FOXO3a-downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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References (38)
  • CDYL1-dependent decrease in lysine crotonylation at DNA double-strand break sites functionally uncouples transcriptional silencing and repair
    Year 2022
    Journal MOLECULAR CELL
    Authors Enas R. Abu-Zhayia, et al.
    Applications Unspecified
    Reactivity
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Lin Dang, et al.
    Applications Unspecified
    Reactivity
  • HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility
    Year 2022
    Journal Hepatology International
    Authors Cui Xiaohan, et al.
    Applications Unspecified
    Reactivity
  • Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation
    Year 2022
    Journal Cells
    Authors Anna Pezzotta, et al.
    Applications Unspecified
    Reactivity
  • Sodium butyrate mediates histone crotonylation and alleviated neonatal rats hypoxic-ischemic brain injury through gut-brain axis.
    Year 2022
    Journal Frontiers in Microbiology
    Authors Xuejia He, et al.
    Applications Unspecified
    Reactivity
  • Quantitative acetylated proteomics on left atrial appendage tissues revealed atrial energy metabolism and contraction status in patients with valvular heart disease with atrial fibrillation.
    Year 2022
    Journal Frontiers in Cardiovascular Medicine
    Authors Tao Tu, et al.
    Applications Unspecified
    Reactivity
  • Proteome-wide analyses reveal diverse functions of protein acetylation and succinylation modifications in fast growing stolons of bermudagrass (Cynodon dactylon L.)
    Year 2022
    Journal BMC PLANT BIOLOGY
    Authors Zhang Bing, et al.
    Applications Unspecified
    Reactivity
  • Histone crotonylation promotes mesoendodermal commitment of human embryonic stem cells
    Year 2021
    Journal Cell Stem Cell
    Authors Yi Fang, et al.
    Applications Unspecified
    Reactivity
  • Discovery of Dihydro-1,4-Benzoxazine Carboxamides as Potent and Highly Selective Inhibitors of Sirtuin-1
    Year 2021
    Journal JOURNAL OF MEDICINAL CHEMISTRY
    Authors Martin Spinck, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • Acetylation of glucosyltransferases regulates Streptococcus mutans biofilm formation and virulence
    Year 2021
    Journal PLoS Pathogens
    Authors Qizhao Ma, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Zhiqiang Lu, et al.
    Applications Unspecified
    Reactivity
  • Lysine Acetylation in the Proteome of Renal Tubular Epithelial Cells in Diabetic Nephropathy
    Year 2021
    Journal Frontiers in Genetics
    Authors Jiayi Wan, et al.
    Applications Unspecified
    Reactivity
  • Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies
    Year 2021
    Journal Scientific Reports
    Authors Kollenstart, et al.
    Applications Unspecified
    Reactivity
  • Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis
    Year 2021
    Journal Scientific Reports
    Authors Perico, et al.
    Applications Unspecified
    Reactivity
  • Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome
    Year 2021
    Journal AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
    Authors Jessica N, et al.
    Applications Unspecified
    Reactivity
  • Protein Hyperacylation Links Mitochondrial Dysfunction with Nuclear Organization
    Year 2020
    Journal MOLECULAR CELL
    Authors Smestad John, et al.
    Applications Unspecified
    Reactivity
  • LACTB promotes metastasis of nasopharyngeal carcinoma via activation of ERBB3/EGFR-ERK signaling resulting in unfavorable patient survival
    Year 2020
    Journal CANCER LETTERS
    Authors Li-Xia Peng, et al.
    Applications Unspecified
    Reactivity
  • Protein Lysine Acetylation in Ovarian Granulosa Cells Affects Metabolic Homeostasis and Clinical Presentations of Women With Polycystic Ovary Syndrome
    Year 2020
    Journal Frontiers in Cell and Developmental Biology
    Authors Min Zheying, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 couples light-driven body temperature cues to hepatic circadian phase coherence and gluconeogenesis
    Year 2019
    Journal Nature Metabolism
    Authors Liu Zuojun, et al.
    Applications Unspecified
    Reactivity
  • Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Year 2019
    Journal Communications Biology
    Authors Li Yanjian, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran
    Year 2019
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    Authors Shihab U. Sobuz, et al.
    Applications Unspecified
    Reactivity
  • Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription
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    Authors Leonie Kollenstart, et al.
    Applications Unspecified
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  • Sirt6 deacetylase activity regulates circadian rhythms via Per2
    Year 2019
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    Authors Shimin Sun, et al.
    Applications Unspecified
    Reactivity
  • Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis
    Year 2018
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    Authors Joanna Kalucka, et al.
    Applications Unspecified
    Reactivity
  • p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis
    Year 2018
    Journal MOLECULAR CELL
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice
    Year 2018
    Journal EBioMedicine
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response
    Year 2018
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    Authors Miyasato, et al.
    Applications Unspecified
    Reactivity
  • Global Proteome Analysis Links Lysine Acetylation to Diverse Functions in Oryza Sativa
    Year 2018
    Journal PROTEOMICS
    Authors Chao Xue, et al.
    Applications Unspecified
    Reactivity
  • Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
    Year 2018
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xianping Fang, et al.
    Applications Unspecified
    Reactivity
  • Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis
    Year 2017
    Journal MOLECULAR CELL
    Authors Shumeng Liu, et al.
    Applications Unspecified
    Reactivity
  • Large-Scale Identification of Protein Crotonylation Reveals Its Role in Multiple Cellular Functions
    Year 2017
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Wei Wei, et al.
    Applications Unspecified
    Reactivity
  • Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4
    Year 2017
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Md. Fazlul Karim, et al.
    Applications Unspecified
    Reactivity
  • Nitrogen regulator GlnR directly controls transcription of genes encoding lysine deacetylases in Actinobacteria
    Year 2017
    Journal MICROBIOLOGY
    Authors Ying Xu, et al.
    Applications Unspecified
    Reactivity
  • Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome
    Year 2017
    Journal Proteomes
    Authors Kathrin Kusch, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability
    Year 2016
    Journal Nature Communications
    Authors Li Lei, et al.
    Applications Unspecified
    Reactivity
  • YcgC represents a new protein deacetylase family in prokaryotes
    Year 2015
    Journal eLife
    Authors Shun Tu, et al.
    Applications Unspecified
    Reactivity
  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
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