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Anti-Acetyllysine Rabbit pAb
Catalog Number: PTM-105
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB IHC-P IP ChIP

Reactivity: All

Synonyms: Kac

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

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    Anti-Acetyllysine Rabbit pAb (BSA and Azide Free)
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    Applications：
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General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Acetyllysine Rabbit pAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:50 - 1:1000 Human, Mouse, Rat
IP 1:25 - 1:500 Human, Mouse
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.

Cellular location

/

Images
Dot Blot

Peptide/Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided below.
Lane 1: Acetylated peptide library. Lane 2: Acetylated BSA. Lane 3: Propionylated BSA.
Lane 4: Butyrylated BSA. Lane 5: BSA.
WB

Lysate: HeLa cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab: Lane A, PTM-105 pre-adsorbed with BSA; Lane B, PTM-105 pre-adsorbed with acetylated BSA
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple
IHC-P

Tissue: Human colon
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Human liver
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Mouse stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.

Tissue: Rat stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-105.
IP

IP of Hela + sodium butyrate (30 mM, 4 hours) cells extracts
IP Ab incubation condition: PTM-105, 4°C overnight, 1:25, 1:100, 1:300 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: (Left) PTM-152, Anti-H2BK5ac Mouse mAb; (Right) PTM-157, Anti-H3K14ac Mouse mAb; 2 hours at room temperature, 1:1000 dilution
Secondary Ab: Goat Anti-Mouse IgG pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 14 kDa / 15 kDa
Observed band size: 14 kDa / 17 kDa

IP of Mouse liver cells extracts
IP Ab incubation condition: PTM-105, 4°C overnight, 1:25, 1:100 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: (Left) PTM-164, Anti-H4K8ac Mouse mAb; (Right) PTM-157, Anti-H3K14ac Mouse mAb; 2 h at room temperature, 1:1000 dilution
Secondary Ab: Goat Anti-Mouse IgG pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 11 kDa / 15 kDa
Observed band size: 11 kDa / 17 kDa
ChIP

Sample: HeLa cells, serum starvation for 12 hours, followed by incubation with 5 mM sodium butyrate for 24 hours.
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30, LDHA promoter, LDHA, FOXO3a promoter, FOXO3a-downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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References (53)
  • Severe fever with thrombocytopenia syndrome virus induces lactylation of m6A reader protein YTHDF1 to facilitate viral replication
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    Authors LiuBingxin , et al.
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  • ActA-mediated PykF acetylation negatively regulates oxidative stress adaptability of Streptococcus mutans
    Year 2024
    Journal mBio
    Authors Qizhao Ma, et al.
    Applications Unspecified
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  • Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle
    Year 2024
    Journal Nature Communications
    Authors Guo Zihao, et al.
    Applications Unspecified
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  • Fisetin disrupts mitochondrial homeostasis via superoxide dismutase 2 acetylation in pancreatic adenocarcinoma
    Year 2024
    Journal PHYTOTHERAPY RESEARCH
    Authors Yimin Ding, et al.
    Applications Unspecified
    Reactivity
  • Sirtulin–Ypk1 regulation axis governs the TOR signaling pathway and fungal pathogenicity in Cryptococcus neoformans
    Year 2024
    Journal Microbiology Spectrum
    Authors Zhenghua Chai, et al.
    Applications Unspecified
    Reactivity
  • Proteome, lysine acetylome and succinylome identify post-translational modification of STAT1 as a novel drug target in silicosis
    Year 2024
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Tiantian Zhang, et al.
    Applications Unspecified
    Reactivity
  • GCN5 mediates DNA-PKcs crotonylation for DNA double-strand break repair and determining cancer radiosensitivity
    Year 2024
    Journal BRITISH JOURNAL OF CANCER
    Authors Han Yang, et al.
    Applications Unspecified
    Reactivity
  • Histone butyrylation in the mouse intestine is mediated by the microbiota and associated with regulation of gene expression
    Year 2024
    Journal Nature Metabolism
    Authors Gates Leah A., et al.
    Applications Unspecified
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  • Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria
    Year 2023
    Journal PLANT PHYSIOLOGY
    Authors Jia Kun, et al.
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  • Post-translational modification of CDK1–STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties
    Year 2023
    Journal Cell and Bioscience
    Authors Xu Xiaodong, et al.
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  • PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation: PGAM5 deacetylation facilitates liver cancer proliferation
    Year 2023
    Journal ACTA BIOCHIMICA ET BIOPHYSICA SINICA
    Authors Gongyu Fu, et al.
    Applications Unspecified
    Reactivity
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    Year 2023
    Journal iScience
    Authors Si-Yi Xu, et al.
    Applications Unspecified
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  • Insights into the Lysine Acetylome of the Haloarchaeon Haloferax volcanii during Oxidative Stress by Quantitative SILAC-Based Proteomics
    Year 2023
    Journal Antioxidants
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    Applications Unspecified
    Reactivity
  • Hypoxia Increases ATX Expression by Histone Crotonylation in a HIF-2α-Dependent Manner
    Year 2023
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Mengxia Qu, et al.
    Applications Unspecified
    Reactivity
  • CDYL1-dependent decrease in lysine crotonylation at DNA double-strand break sites functionally uncouples transcriptional silencing and repair
    Year 2022
    Journal MOLECULAR CELL
    Authors Enas R. Abu-Zhayia, et al.
    Applications Unspecified
    Reactivity
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
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    Authors Dang Lin, et al.
    Applications Unspecified
    Reactivity
  • HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility
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    Journal Hepatology International
    Authors Cui Xiaohan, et al.
    Applications Unspecified
    Reactivity
  • Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation
    Year 2022
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    Authors Anna Pezzotta, et al.
    Applications Unspecified
    Reactivity
  • Sodium butyrate mediates histone crotonylation and alleviated neonatal rats hypoxic-ischemic brain injury through gut-brain axis.
    Year 2022
    Journal Frontiers in Microbiology
    Authors Xuejia He, et al.
    Applications Unspecified
    Reactivity
  • Quantitative acetylated proteomics on left atrial appendage tissues revealed atrial energy metabolism and contraction status in patients with valvular heart disease with atrial fibrillation.
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    Applications Unspecified
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    Applications Unspecified
    Reactivity
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    Authors Smestad John, et al.
    Applications Unspecified
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    Year 2020
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    Authors Li-Xia Peng, et al.
    Applications Unspecified
    Reactivity
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    Year 2020
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    Authors Min Zheying, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 couples light-driven body temperature cues to hepatic circadian phase coherence and gluconeogenesis
    Year 2019
    Journal Nature Metabolism
    Authors Liu Zuojun, et al.
    Applications Unspecified
    Reactivity
  • Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Year 2019
    Journal Communications Biology
    Authors Li Yanjian, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran
    Year 2019
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
    Authors Shihab U. Sobuz, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Leonie Kollenstart, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Shimin Sun, et al.
    Applications Unspecified
    Reactivity
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    Year 2018
    Journal Cell Metabolism
    Authors Joanna Kalucka, et al.
    Applications Unspecified
    Reactivity
  • p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis
    Year 2018
    Journal MOLECULAR CELL
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice
    Year 2018
    Journal EBioMedicine
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response
    Year 2018
    Journal Scientific Reports
    Authors Miyasato Yoshikazu, et al.
    Applications Unspecified
    Reactivity
  • Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
    Year 2018
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xianping Fang, et al.
    Applications Unspecified
    Reactivity
  • Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis
    Year 2017
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    Authors Shumeng Liu, et al.
    Applications Unspecified
    Reactivity
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    Year 2017
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    Authors Wei Wei, et al.
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    Year 2017
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    Year 2017
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    Year 2015
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    Applications Unspecified
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  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2014
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
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