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Recombinant Rabbit Monoclonal
Anti-alpha E-Catenin Rabbit mAb
Catalog Number: PTM-6174
This item is discontinued.
Please see alternative products below.

Clone Number: JRMR-10584-41

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Catenin alpha-1, CTNNA1

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Catenin alpha-1, CTNNA1
UniProt ID

Q99697

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation.

Cellular location

Membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours atroom temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate: 1: HT-29, 2: A431
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 100 kDa
Observed band size: 95-130 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HT-29 cell lysate, the CTNNA1
Rabbit mAb (PTM-6174) pre-adsorbed with 3μM of the synthetic peptides; 2: HT-29 cell lysate, the CTNNA1 Rabbit mAb (PTM-6174)with no peptide blocking.
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 100 kDa
Observed band size: 95-130 kDa

Research Use

For research use only, not for use in diagnostic procedures.