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Recombinant Rabbit Monoclonal
Anti-alpha Tubulin Rabbit mAb
Catalog Number: PTM-5442
$ 130

Clone Number: JRMR-10062-14H2L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF FC

Reactivity: Human, Mouse, Rat

Synonyms: Alpha-tubulin 1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Alpha-tubulin 1
UniProt ID

P68366

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:5000 Human, Mouse, Rat
IHC-P 1:50 - 1:1000 Human, Mouse
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Cellular location

Cytoskeleton

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1:Jurkat, 2:C2C12, 3:PC-12, 4:COS-7, 5:Human heart, 6:Mouse heart, 7:Rat heart
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 50 kDa
Observed band size: 50 kDa

IHC-P

Tissue: Human cerebellum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:50
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5442

Tissue: Human melanoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5442

Tissue: Mouse brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:50
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5442

ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5442

FC

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5442

Research Use

For research use only, not for use in diagnostic procedures.

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References (6)
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  • Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice
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  • Single-cell transcriptome reveals cellular hierarchies and guides p-EMT-targeted trial in skull base chordoma
    Year 2022
    Journal Cell Discovery
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