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Anti-Benzoyllysine Mouse mAb
Catalog Number: PTM-762
$ 435

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications: WB IHC-P

Reactivity: All

Synonyms: Kbz

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbz
UniProt ID

/

Immunogen Benzoylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Benzoyllysine Mouse mAb detects proteins with benzoylated lysine residues. This pan antibody recognizes benzoylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine benzoyllysine is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine benzoylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes.

Cellular location

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Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Benzoylated peptide library. Lane 2: Phosphorylated tyrosine peptide library.
Lane 3: 3-Nitrotyrosine peptide library. Lane 4: Unmodified peptide library.

WB

Lysates: HeLa, N2a, and HepG2 cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

IHC-P

Tissue: Human placenta
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-762.

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-762.

Tissue: Mouse liver
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-762.

Tissue: Rat cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-762.

Research Use

For research use only, not for use in diagnostic procedures.

References (5)
  • Global profiling of regulatory elements in the histone benzoylation pathway
    Year 2022
    Journal Nature Communications
    Authors Wang Duo, et al.
    Applications Unspecified
    Reactivity
  • Histone benzoylation serves as an epigenetic mark for DPF and YEATS family proteins
    Year 2021
    Journal NUCLEIC ACIDS RESEARCH
    Authors Ren Xiangle, et al.
    Applications Unspecified
    Reactivity
  • Isonicotinylation is a histone mark induced by the anti-tuberculosis first-line drug isoniazid
    Year 2021
    Journal Nature Communications
    Authors Jiang Yuhan, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • Lysine benzoylation is a histone mark regulated by SIRT2
    Year 2018
    Journal Nature Communications
    Authors Huang He, et al.
    Applications Unspecified
    Reactivity