中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant
Anti-beta Actin Mouse mAb
Catalog Number: PTM-5018
$ 125

Clone Number: 10178

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat, A.thaliana, Rice

Synonyms: ACTB

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms ACTB
UniProt ID

P60709

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human, Mouse, Rat, Arabidopsis thaliana, Rice
IHC-P 1:50 - 1:100 Human, Mouse
ICC/IF 1:50 - 1:100 Human, Mouse
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

Cellular location

Cytoskeleton, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: 1: HeLa, 2: HepG2, 3: Neuro-2a, 4: EL4.IL-2, 5: BRL
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted band size: 41 kDa
Observed band size: 45 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: 1: Arabidopsis leaves, 2: Rice leaves
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 41 kDa
Observed band size: 45 kDa
IHC-P

Tissue: Human colon
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5018

Tissue: Mouse colon
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5018
ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5018

Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5018

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（7）
    $ 130
    Recombinant Rabbit Monoclonal
    Anti-beta Actin Rabbit mAb
    Catalog #
    PTM-5028
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（7）
    $ 130
    Recombinant Rabbit Monoclonal
    Anti-beta Actin Rabbit mAb
    Catalog #
    PTM-5436
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（7）
    $ 130
    Recombinant Rabbit Monoclonal
    Anti-beta Actin Rabbit mAb
    Catalog #
    PTM-5455
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (5)
  • Cold atmospheric plasma restores skewed macrophage polarization in triple negative breast cancers via enhancing KAT6A acetylation
    Year 2024
    Journal FREE RADICAL BIOLOGY AND MEDICINE
    Authors Kaiyuan Zhu, et al.
    Applications Unspecified
    Reactivity
  • Lactylome Analysis Reveals Potential Target Modified Proteins in the Retina of Form-deprivation Myopia
    Year 2024
    Journal iScience
    Authors Jiaojiao Feng, et al.
    Applications Unspecified
    Reactivity
  • Mechanistic role of RND3-regulated IL33/ST2 signaling on cardiomyocyte senescence
    Year 2024
    Journal LIFE SCIENCES
    Authors Linxu Wu, et al.
    Applications Unspecified
    Reactivity
  • PP2C phosphatases Ptc1 and Ptc2 dephosphorylate PGK1 to regulate autophagy and aflatoxin synthesis in the pathogenic fungus Aspergillus flavus
    Year 2023
    Journal mBio
    Authors Zhuo Zhu, et al.
    Applications Unspecified
    Reactivity
  • Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii
    Year 2022
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.