Background
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells.
Cellular location
Cytoskeleton
Clone Number: JRMR-64
Host: Rabbit Clonality: Recombinant Monoclonal
Applications： WB IHC-P ICC/IF FC IP
Reactivity: Human, Mouse, Rat
Synonyms: Actin, cytoplasmic 1
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | Actin, cytoplasmic 1 |
| UniProt ID | |
| Immunogen | Recombinant human beta actin fragment |
| MW (kDa) | 42 |
| Specificity | Anti-beta Actin Rabbit mAb recognizes endogenous level of beta actin. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat |
| IHC-P | 1:50 - 1:100 | Human |
| ICC/IF | 1:50 - 1:100 | Mouse |
| FC | 1:50 - 1:100 | Human, Mouse |
| IP | 1:25 - 1:100 | Human |
| Purity | Protein A purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells.
Cellular location
Cytoskeleton
| WB | |
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Blocking buffer: 5% NFDM/TBST |
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Blocking buffer: 5% NFDM/TBST |
| IHC-P | |
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Tissue: Human colon |
| ICC/IF | |
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Cell line: HeLa |
| FC | |
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Cell line: HeLa |
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Cell line: NIH/3T3 |
| IP | |
|---|---|
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IP of HeLa cells extracts |
Research Use
For research use only, not for use in diagnostic procedures.
