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Recombinant
Anti-BRG1 Mouse mAb
Catalog Number: PTM-5826
$ 280

Clone Number: 11483

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Rat

Synonyms: BRG1, BAF190A, SNF2B, SNF2L4

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2b/kappa
Conjugate Unconjugated
Synonyms BRG1, BAF190A, SNF2B, SNF2L4
UniProt ID

P51532

Immunogen
MW (kDa) 185
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Rat
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Transcription activator BRG1 also known as ATP-dependent chromatin remodeler SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44.

Cellular location

Nucleus, Secreted protein

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: HepG2, 3: C6, 4: Jurkat
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 185 kDa
Observed band size: 220 kDa

Research Use

For research use only, not for use in diagnostic procedures.